DNA-Sequence Determination of Exon 2 of a Novel HLA-DPB1 Allele, HLA-DPB1*0403

Details

• Personal Author:
  Ensey JS ; Frye BL ; Weston A
• Description:
  Sequence determination using HLA-DPB1 allele-specific primers for a DNA sample donated by an African-American individual revealed the presence of a novel haplotype. This new allele was found as a heterozygote together with HLA-DPB1*0402. The new allele was similar to HLA-DPB1*1601, however, it varied in two single nucleotide polymorphisms resulting in alanine residues at positions 55 and 56 of the mature protein rather than aspartic acid and glutamic acid, respectively. Allele-specific DNA-sequence determination was verified by sequence determination in forward and reverse directions after cloning in pCR2.1. This cloning strategy resulted in DNA products representing 19 clones confirming the novel allele (GenBank accession number AY823995 and is now listed in the IMGT/HLA database as HLA-DPB1*0403) and 17 clones representing HLA-DPB1*0402. [Description provided by NIOSH]
• Subjects:
  Demography Genetics Nucleotides Occupational Health Race Factors Safety
• Keywords:
  Demographic-characteristics DNA-adducts Gene-mutation Genetic-factors Molecular-biology Molecular-structure Racial-factors Sampling
• ISSN:
  1042-5179
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  235-236
• Volume:
  16
• Issue:
  3
• NIOSHTIC Number:
  nn:20027632
• Citation:
  DNA Seq 2005 Jun; 16(3):235-236
• Contact Point Address:
  A. Weston, Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505-2888, USA
• Email:
  agw8@cdc.gov
• Federal Fiscal Year:
  2005
• Peer Reviewed:
  True
• Source Full Name:
  DNA Sequence
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:35a375cf59acdf9e243fe0cd3f20899c9fa73b7de6727bd7620f1611ccc62fe10671e8b9f99641749f9fabd66bc57c200683437bb6427d5187331f15ff0c4512
• Download URL:
  https://stacks.cdc.gov/view/cdc/197608/cdc_197608_DS1.pdf
• File Type:
  [PDF - 224.36 KB ]