Topographical Organization of the N-Terminal Segment of Lung Pulmonary Surfactant Protein B (SP-B 1–25) in a Phospholipid Bilayer as Determined by Fluorescence Quenching

Details

• Personal Author:
  Demchuk, Eugene ; Rao MK ; Wang Y
• Description:
  The location and depth of each residue on the phospholid bilayer (PB) was determined by fluorescence quenching with synthesized single residue substituted peptides that were rconstituted into DPPC-enriched liposomes. The single residue subsitutions in peptides were either aspartate or tryptophan. Asparate was subsequently labeled with the NCD-4 flurophore. The spin-labeled compounds, 5-DSA, 7-DSA, 12-DSA, CAT-16, and CAT-1 were used in quenching experiments. Our observations indicated that residues 1-6 are located at the surface of PB; residues 7-9 are embedded in PB; residues 10-22 are involved in an amphipathic alpha-helix with its axis somewhat parallel to the surface of PB; residues 23-25 reside at the surface. Effects of the inter-molecule disulfide bond formation in the SP-B 1-25 dimer were also investigated. The data suggest that hydrophobic sides of the amphipathic helixes face each other forming a hydrophobic domain. The environment-sepcific conformational liability in this hydrophobic domain may explain the key impact of SP-B on the phospholipid transport from bi- to mono-layer and in modulating the cell inflammatory response during the respiratory distress syndrome conditions. [Description provided by NIOSH]
• Subjects:
  Lipids Lung Occupational Health Respiratory System Safety
• Keywords:
  Cellular-function Cellular-reactions Lung-function Peptides Phospholipids Pulmonary-function Pulmonary-system
• ISSN:
  0006-3495
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  84
• Issue:
  2
• NIOSHTIC Number:
  nn:20027490
• Citation:
  Biophys J 2003 Feb; 84(2)(Pt 2):54a
• Contact Point Address:
  1095 Willowdale Road, Morgantown, WV, 26505
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  False
• Part Number:
  2
• Source Full Name:
  Biophysical Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7c6096dbc756ed65b1db0dc46bbb6d3cd8fea075b940410f07f4f4a10613252c0367b546dc299661dadd846c5574755ea323785a7d528bea4b0ed44eb961b96e
• Download URL:
  https://stacks.cdc.gov/view/cdc/197584/cdc_197584_DS1.pdf
• File Type:
  [PDF - 191.20 KB ]