Determination of Benzo[a]pyrene-DNA Adducts by Solid-Matrix Phosphorescence

Details

• Personal Author:
  Hurtubise RJ ; Li M ; Weston A
• Description:
  It has been demonstrated for the first time that linear relationships can be obtained between the solid-matrix phosphorescence (SMP) and the percent modification of benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE)-DNA adducts. The samples of DNA were modified with BPDE at levels of 5.0 × 10-3, 1.0 × 10-3, 5.0 × 10-4, 1.0 × 10-4, 5.0 × 10-5, and 1.0 × 10-5%. In addition, the changes in the SMP intensities of a given percentage of DNA adduct were investigated as a function of sample size, and linear relationships were also acquired. With the different percentages of modified DNA, very good reproducibility of the SMP signals was obtained. Data were acquired with both Whatman 1PS paper and 30% TlNO3/sodium acetate as solid matrixes. The limit of detection for the BPDE-DNA adducts was 2 adducts in 107 bases for both Whatman 1PS paper and 30% TlNO3/sodium acetate. In addition, it was shown that it would be important to develop a standard procedure for the preparation of the BPDE-DNA samples if different batches of DNA were used in the preparation procedure. [Description provided by NIOSH]
• Subjects:
  Occupational Health Safety
• Keywords:
  DNA-adducts Phosphorescence-polarization Sampling Sampling-methods Sodium-compounds
• ISSN:
  0003-2700
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  New Jersey ; OSHA Region 2 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  71
• Issue:
  20
• NIOSHTIC Number:
  nn:20027323
• Citation:
  Anal Chem 1999 Oct; 71(20):4679-4683
• Contact Point Address:
  NIOSH/CDC/HELD/TMBB, 1095 Willowdale Road, MS 3014, Morgantown, West Virginia 26505
• CAS Registry Number:
  Benzo[a]pyrene-7,8-dihydrodiol (CAS RN 13345-25-0) ; Sodium acetate (CAS RN 127-09-3)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Analytical Chemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3c8bf92c727d20933082a4977515664a69b34631dd6edc34cca27484b743abb8f64953d0a0a7a3f26f34ffbbea405113cfcc7acc172f0d8f0e213306d3c667e8
• Download URL:
  https://stacks.cdc.gov/view/cdc/197545/cdc_197545_DS1.pdf
• File Type:
  [PDF - 87.34 KB ]