Cytotoxic and Genotoxic Effects of Single Wall Carbon Nanotube Exposure on Human Keratinocytes and Bronchial Epithelial Cells

Details

• Personal Author:
  Arepalli S ; Castranova, Vincent ; Gandelsman VZ ; Gorelik O ; Keshava N ; Kisin E ; Murray AR ; Shvedova AA ; Arepalli S ; Castranova, Vincent ; Gandelsman VZ ; Gorelik O ; Keshava N ; Kisin E ; Murray AR ; Shvedova AA
• Description:
  Carbon nanotubes are new members of carbon allotropes similar to fullerenes and graphite. Because of their unique electrical, mechanical and thermal properties, carbon nanotubes are being evaluated for novel applications in electronics, aerospace and computer industries. Occupational exposure to graphite and carbon materials has been associated with increased incidence of skin and lung diseases, e.g.. carbon fiber dermatitis, hyperkeratosis, naevl, asthma, pneumoconiosis and cancer. However, the potential toxicity and mechanisms of action of single wall carbon nanotubes (SWCNT) have yet to be determined. The present talk will. be focused on adverse effects of SWCNT studied in cell culture models - human epidermal keratinocytes (HaCaT) and bronchial epithelial cells (BEAS-2B). SWCNT caused ultra-structural and morphological changes, loss of cell integrity and apoptosia. accelerated oxidative stress indicated by the formation of carbon-centered and hydroxyl radicals, accumulation of peroxidative products, and depletion of antioxidants in the cells. Transcriptional response to SWCNT in BEAS2 and HaCaT cells detected by microarray analysis revealed alterations of a number of genes including those involved in oxidative stress. The data suggest that exposure to SWCNT may lead to dermal and pulmonary toxicity, and identify oxidative stress as one of potentially important mechanisms of cell injury. [Description provided by NIOSH]
• Subjects:
  Antioxidants Cytotoxins Genetics Occupational Health Respiratory Tract Diseases Safety Skin Diseases
• Keywords:
  Antioxidation Cell-alteration Cell-biology Cell-damage Cellular-function Cellular-reactions Cellular-respiration Cytotoxic-effects Cytotoxicity Genes Genotoxic-effects Nanotechnology Pulmonary-system-disorders Respiratory-system-disorders Skin-disorders

  More +
• ISSN:
  0065-7727
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings ; Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  227
• NIOSHTIC Number:
  nn:20025959
• Citation:
  Abstr Pap - Am Chem Soc 2004 Mar; 227(Pt 1):IEC-20
• Contact Point Address:
  NIOSH, HELD, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  ats1@cdc.gov
• Federal Fiscal Year:
  2004
• NORA Priority Area:
  Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease ; Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
• Peer Reviewed:
  False
• Part Number:
  1
• Source Full Name:
  Abstracts of papers - American Chemical Society
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:eea7a3274ba79f4ac161af9b0c7a0451a49c3cbbfd682c9945999771a64790a018c367200b9f1927b6f2ef36d1e102e1634c00264c6aff6f842e1a174348244b
• Download URL:
  https://stacks.cdc.gov/view/cdc/197114/cdc_197114_DS1.pdf
• File Type:
  [PDF - 294.73 KB ]