Characterization of Phagolysosomal Simulant Fluid for Study of Beryllium Aerosol Particle Dissolution

Details

• Personal Author:
  Breysse PN ; Day, Gregory A. ; Guilmette RA ; Hoover, Mark D. ; Scripsic RC ; Stefaniak, Aleksandr B.
• Description:
  A simulant of phagolysosomal fluid is needed for beryllium particle dissolution research because intraphagolysosomal dissolution is believed to be a necessary step in the cellular immune response associated with development of chronic beryllium disease. Thus, we refined and characterized a potassium hydrogen phthalate (KHP) buffered solution with pH 4.55, termed phagolysosomal simulant fluid (PSF), for use in a static dissolution technique. To characterize the simulant, beryllium dissolution in PSF was compared to dissolution in the J774A.1 murine cell line. The effects of ionic composition, buffer strength, and the presence of the antifungal agent alkylbenzyldimethylammonium chloride (ABDC) on beryllium dissolution in PSF were evaluated. Beryllium dissolution in PSF was not different from dissolution in the J774A.1 murine cell line (p=0.78) or from dissolution in another simulant having the same pH but different ionic composition (p=0.73). A buffer concentration of 0.01-M KHP did not appear adequate to maintain pH under all conditions. There was no difference between dissolution in PSF with 0.01-M KHP and 0.02-M KHP (p=0.12). At 0.04-M KHP, beryllium dissolution was increased relative to 0.02-M KHP (p=0.02). Use of a 0.02-M KHP buffer concentration in the standard formulation for PSF provided stability in pH without alteration of the dissolution rate. The presence of ABDC did not influence beryllium dissolution in PSF (p=0.35). PSF appears to be a useful and appropriate model of in vitro beryllium dissolution when using a static dissolution technique. In addition, the critical approach used to evaluate and adjust the composition of PSF may serve as a framework for characterizing PSF to study dissolution of other metal and oxide particles. [Description provided by NIOSH]
• Subjects:
  Aerosols Beryllium Chlorine Immune System Lung Occupational Health Phosphoric Acids Phthalic Acids Potassium Compounds Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Aerosol-particles Alveolar-cells Ammonium-compounds Author Keywords: Alveolar Macrophage Phagolysosome Beryllium-compounds Beryllium-disease Cellular-reactions Chlorides Chronic Beryllium Disease Dissolution Immune-reaction In-vitro-studies Oxides Phagocytic-activity Phosphates Phthalates Physiological-chemistry Potassium-compounds Respiratory-system-disorders Simulant Stimulants

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• ISSN:
  0887-2333
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; New Mexico ; OSHA Region 3 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  123-134
• Volume:
  19
• Issue:
  1
• NIOSHTIC Number:
  nn:20025800
• Citation:
  Toxicol In Vitro 2005 Feb; 19(1):123-134
• Contact Point Address:
  A.B. Stefaniak, National Institute for Occupational Safety and Health, Division of Respiratory Disease Studies, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  astefaniak@cdc.gov
• CAS Registry Number:
  Beryllium (CAS RN 7440-41-7) ; Beryllium oxide (CAS RN 1304-56-9) ; Potassium hydrogen phthalate (CAS RN 877-24-7)
• Federal Fiscal Year:
  2005
• NORA Priority Area:
  Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
• Performing Organization:
  Johns Hopkins University, Baltimore, Maryland
• Peer Reviewed:
  True
• Start Date:
  20010601
• Source Full Name:
  Toxicology in Vitro
• End Date:
  20040531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c4487b7e8ca21ac3482feabfd1491746ae46320d6b3d7767973ce80dea0c1bb79df7c54e9d77a91a6be7f7a1ab44ad3be75ad0576cd53255ece864faca8f27bd
• Download URL:
  https://stacks.cdc.gov/view/cdc/197021/cdc_197021_DS1.pdf
• File Type:
  [PDF - 373.08 KB ]