Comparison of Urinary 8-Hydroxydeoxyguanosine, Isoprostane and TBARS for Assessing Oxidative Stress in Smokers

Details

• Personal Author:
  Cheever KN ; Marlow, Kate L. ; Mathias PI ; Toraason MA
• Description:
  Measurements of oxidative stress are used increasingly as biomarkers of biologically effective dose for assessing environmental and occupational exposures. Three frequently used indices of oxidative stress 8-hydroxydeoxy-guanosine (8-OHdG), thiobarbiruric acid reactive substances (TBARS), and the isoprostane, 8-epiprostaglandin F2alpha (8-epi-PGF) were measured in the urine (creatinine corrected) of 38 males employed in the construction industry. The objective was to determine the inter-relationship of these biomarkers and their utility in assessing the effect of smoking, a known inducer of oxidative stress. Urine samples were obtained at the start of a typical workweek, and again at the end of that workweek. Urinary 8-OHdG is considered an index of oxidative DNA damage repair. Mean 8-OHdG values at both the start and end of the week tended to be higher, bur not significantly higher, in non-smokers than in smokers. TBARS and 8-epi-PGF were used to assess lipid peroxidation. TBARS and 8-epi-PGF were greater in smokers than in non-smokers, bur the increase was significant only for the end-of-week 8-epi-PGF values. 8-epi-PGF and TBARS declined non-significantly during the workweek, whereas 8-OHdG increased, and the increase was significant within the smoking group. Although regression analysis did not reveal any significant correlations among the three biomarkers, TBARS paralleled 8-epi-PGF in terms of differences between smokers and nonsmokers and changes during the workweek. In contrast, intergroup differences and temporal changes in 8-OHdG were counter to TBARS and 8-epi-PGF. For the present population, urinary 8-epi-PGF was the most sensitive biomarker of oxidative stress associated with smoking. [Description provided by NIOSH]
• Subjects:
  Biomarkers Construction Industry Demography Environmental Exposure Occupational Exposure Occupational Health Safety Sex Characteristics Smokers Urine
• Keywords:
  Construction-industry Construction-workers Demographic-characteristics Environmental-exposure Occupational-exposure Sex-factors Smoking Urinalysis
• ISSN:
  1096-6080
• Publisher:
  Oxford University Press
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  85
• Volume:
  60
• Issue:
  1
• NIOSHTIC Number:
  nn:20025755
• Citation:
  Toxicologist 2001 Mar; 60(1):85
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 40th Annual Meeting, March 25-29, 2001, San Francisco, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:87ed578dba9146620d5a53f65244084853b2d7ed4898db18d3893047b58156734279c4135993bdf24d7d1f9e5002163e8d0920aad940d0ab5bb5c02e3cc29abf
• Download URL:
  https://stacks.cdc.gov/view/cdc/196999/cdc_196999_DS1.pdf
• File Type:
  [PDF - 124.72 KB ]