Development and Evaluation of a Rapid, Sensitive, and Specific Immunochromatographic Lateral Flow Assay for Anthrax Protective Antigen Immunity Status

Details

• Personal Author:
  Biagini, Raymond E. ; MacKenzie, Barbara A. ; Robertson SR ; Sammons DL ; Smith JP ; Snawder, John E. ; Striley CAF
• Description:
  Evidence from animals suggests that anti-protective antigen (PA) igG from vaccination with Anthrax Vaccine Adsorbed (AVA) is protective against B. antrhacis infection. Measurement of anti-PA igG in human sera can be performed using their Enzyme-Linked Immunosorbent Assay (ELISA) or Fluorescent Covalent Microsphere Immunoassay, as reported previously. Both these methods are laboratory based. We describe the development of a rapid lateral flow immunochromatographic assay (LFIA) test kit for the measurement of anti-PA IgG in serum or whole blood (50 ul sample) using colloidal gold nanoparticles as the detection reagant and an internal control. Using sera from 19 AVA vaccines (anti-PA igG range, 2,4 - 267 ug/ml), 10 controls and PA-supplemented whole blood, we demonstrated the LFIA had a sensitivity of -2 ug/ml anti-PA IgG in sera and -14 ug/ml anti-PA IgG in whole blood. Pre-adsorption of sera with PA yielded negative anti-PA LFIAs. Internal controls were positive for all tests. The diagnostic sensitivity and specificity of the assay (for human sera) were 100% using FCMIA anti-PA IgG as standard. This kid has utility for determining the immune status of individuals (military personnel, decontamination workers) vaccinated with AVA or possibly exposed to sub-clinical levels of B. anthracis. [Description provided by NIOSH]
• Subjects:
  Animals Antigens Blood Hematologic Tests Laboratories Occupational Health Safety
• Keywords:
  Animal-studies Blood-samples Blood-sampling Laboratory-animals Nanotechnology Vaccines
• ISSN:
  0002-9637
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  49
• Volume:
  71
• NIOSHTIC Number:
  nn:20025669
• Citation:
  Am J Trop Med Hyg 2004 Oct; 71(Suppl 4):49
• Federal Fiscal Year:
  2005
• Peer Reviewed:
  False
• Source Full Name:
  The American Journal of Tropical Medicine and Hygiene. Abstracts Of The 53rd Annual Meeting Of The American Society Of Tropical Medicine And Hygiene: Fontainebleau Hilton, Miami Beach, Florida U.S.A., November 7-11, 2004
• Supplement:
  4
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:885ccc54ffbc5d23d3ba39669fd2245f439746c4a28f9ec9b4194d0360c77e194c6336c65a70332dd26e38bed9e913c3e28258eb2e890cb39e93f77386c810cf
• Download URL:
  https://stacks.cdc.gov/view/cdc/196940/cdc_196940_DS1.pdf
• File Type:
  [PDF - 40.37 KB ]