Characterization of the In Vitro Unscheduled DNA Synthesis Assay in Primary Lung Cells of the Rat

Details

• Personal Author:
  Ong T ; Stewart JD ; Whong, Wen-Zong
• Description:
  The in vitro unscheduled DNA synthesis (UDS) assay has been evaluated in rat primary lung cells with known genotoxicants. The autoradiographic method was employed to detect UDS in both alveolar macrophages and primary pulmonary cells. Data of a time course study revealed that a high radioactive labeling of DNA repair was achieved after a 16-h incubation with [3H]thymidine. Coupled with low serum (1%), hydroxyurea at the concentration of 20 mM inhibited regular DNA synthesis in primary lung cells in a satisfactory manner (81-88% inhibition). With this protocol, a dose-related increase in UDS was induced by N-methyl-N'-nitro-N-nitrosoguanidine and 2-aminoanthracene in both rat alveolar macrophages and primary lung cells. The results suggest that primary rat lung cells in culture possess DNA-repair ability and that the UDS assay may be useful for assessing the pulmonary genotoxic effect of chemicals in this cell system. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Lung Occupational Health Respiratory System Safety
• Keywords:
  Alveolar Macrophages Animal-studies Author Keywords: DNA Repair Cell-function Cell-transformation Cellular-function Genotoxic-effects In-vitro-studies Laboratory-animals Lung-cells Primary Lung Cells Pulmonary-system Rat UDS Unscheduled DNA Synthesis

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• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  51-55
• Volume:
  262
• Issue:
  1
• NIOSHTIC Number:
  nn:20025497
• Citation:
  Mutat Res 1991 Jan; 262(1):51-55
• Contact Point Address:
  Dr. Wen-Zong Whong, Microbiology Section, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888 (U.S.A.)
• Federal Fiscal Year:
  1991
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:83bf380df3f61cc7d9383a10a54ec64c2ede4188fae1baa0fb2ef39c7cf64a870a5be3f1374d966a0fc6f49375bdde7b9219cd2a9d125b2092e12ee8a995d9d5
• Download URL:
  https://stacks.cdc.gov/view/cdc/196837/cdc_196837_DS1.pdf
• File Type:
  [PDF - 349.74 KB ]