Expression Profile of Eukaryotic Translation Factors in Human Cancer Tissues and Cell Lines

Details

• Personal Author:
  Joseph P ; Lei Y-X ; O'Kenrick CM ; Ong T-M ; Othumpangat S ; Yuan B-Z
• Description:
  Several studies have demonstrated the overexpression of certain eukaryotic translation factors in human cancer cell lines and in malignant tissues. In this study, with human cancer cell lines derived from lungs, breast, prostate, and skin, we have examined the expression profile of 36 translation factors consisting of 27 initiation factors, 8 elongation factors, and 1 termination factor. Translation initiation factors 2C2 and 4E1 and translation elongation factors 1A2 and 1 were found overexpressed (2- to 2000-fold) in many of the cancer cell lines compared to their corresponding normal cell lines. Among the translation factors analyzed, translation elongation factor 1A2 exhibited the most significant alteration in expression: 10- to 2000-fold overexpression was noticed in nine out of ten cancer cell lines analyzed. Whether the overexpression of translation elongation factor 1A2 can be used as a potential tumor marker was tested with the cancer profiling array (BD Biosciences, Palo Alto, CA) consisting of 241 paired cDNA samples generated from 13 different cancer/noncancer tissue types. Overexpression of translation elongation factor 1A2 was noticed in several tumor tissue samples, most notably in the human colon cancer samples which exhibited at least a twofold overexpression among 35% of the samples analyzed. Besides colon, tumor samples derived from lungs, kidney, rectum, and ovary also exhibited more than a twofold overexpression of translation elongation factor 1A2 in at least 20% of the samples analyzed. These results indicate that human carcinogenesis is often associated with alterations in the expression of various translation factors especially the overexpression of eukaryotic translation elongation factor 1A2. [Description provided by NIOSH]
• Subjects:
  Carcinogens Neoplasms Occupational Health Safety
• Keywords:
  Author Keywords: Translation Factors Cancer Cancer-rates Carcinogenesis Carcinogenicity Cell-function Cell-growth Cellular-function Cellular-reactions Cellular-structures Expression Profile Malignancy Molecular Marker

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• ISSN:
  0899-1987
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  171-179
• Volume:
  40
• Issue:
  3
• NIOSHTIC Number:
  nn:20025264
• Citation:
  Mol Carcinog 2004 Jul; 40(3):171-179
• Contact Point Address:
  Pius Joseph, MS 3014, Molecular Carcinogenesis Laboratory, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
• Federal Fiscal Year:
  2004
• NORA Priority Area:
  Research Tools and Approaches: Cancer Research Methods
• Peer Reviewed:
  True
• Source Full Name:
  Molecular Carcinogenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3db40f45b31554024f7b6a7b4b944f2e27594a911fbdb935f32fe3287e52f608a73015b612078d0346da706cc1ad609cb55c70ff6958883e470090fcb1d6ede2
• Download URL:
  https://stacks.cdc.gov/view/cdc/196717/cdc_196717_DS1.pdf
• File Type:
  [PDF - 168.52 KB ]