Toxicological Assessment of Diesel-Methanol-Water Emulsion [Purinox (All Weather) Generation 2 Fuel] Exhaust Emissions

Details

• Personal Author:
  Daly I ; Gudi R ; Kraska R ; McDonald JD ; Mercieca M ; O'Callaghan J ; Reed MD ; Ronsko N ; Seilkop S ; Wagner V
• Description:
  A type of fuel shown to decrease combustion emissions vs. traditional diesel is the diesel emulsion. Utilizing a collaborative approach, a toxicological assessment of the exhaust of PuriNOx fuel emulsion was recently conducted. Whole exhaust from one of two, 2002 model Cummins 5.9L ISB engines was diluted to exposure levels of 125, 250, and 500 ug particulate matter (PM)/m 3. The engines were operated on a repeating EPA heavy-duty certification cycle. F344 rats were housed in Hazleton H2000 exposure chambers and exposed to exhaust 6 h/day, 5 days/wk for the first 11 weeks and 7 days a week thereafter. Exposure days ranged from 58-70 days. Exposure- start was staggered to account for multiple health endpoints. Toxicological assays were conducted during and following exposures and subsequent to a recovery period. General toxicity (body weight, organ weight, clinical pathology, and histopathology), neurotoxicity (glial fibrillary acidic protein assay, GFAP), genotoxicity (Ames, micronucleus, sister chromatid exchange), and reproduction and development were assessed. Effects observed were mild. Exhaust was not associated with neurotoxicity, reproductive/developmental toxicity, or in vivo genotoxicity. Small decreases in serum cholesterol were noted in males and females of the high level exposure. PM accumulation within alveolar macrophages was noted in all exposure groups. Other sporadic statistically significant responses were observed but not clearly treatment related. Exhaust subfractions induced mutagenic responses in S. typh. Based on the cholesterol results, it can be concluded that the 250 ug/m 3 exposure level was the NOAEL. In general, these observations were consistent with rodent and bacteria exposure to petroleum diesel exhaust. [Description provided by NIOSH]
• Subjects:
  Bacteria Dust Fuel Oils Nervous System Occupational Health Petroleum Safety Toxicology Vehicle Emissions
• Keywords:
  Bacterial-dusts Combustion-engines Diesel-exhausts Exposure-levels Fuels Genotoxic-effects In-vivo-studies Neurotoxicity
• ISSN:
  1096-6080
• Publisher:
  Society of Toxicology
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Maryland ; New Jersey ; New Mexico ; North Carolina ; Ohio ; OSHA Region 2 ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 5 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  78
• NIOSHTIC Number:
  nn:20025142
• Citation:
  Toxicologist 2004 Mar; 78(S-1):285
• Federal Fiscal Year:
  2004
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 43nd Annual Meeting and ToxExpo, March 21-25, 2004, Baltimore, Maryland
• Supplement:
  S-1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:df1dccae5c44a7be0073c9f85c02581709797738e52a652d5926a5040c83c5b50090ce022c936d2e51507bf1455f77ed0828d655e7420f93d6304677ebedceab
• Download URL:
  https://stacks.cdc.gov/view/cdc/196632/cdc_196632_DS1.pdf
• File Type:
  [PDF - 57.63 KB ]