Comparative Cytotoxic Effects of Crocidolite and Its Non-Asbestiform Polymorph on Rat Alveolar Macrophages

Details

• Personal Author:
  Castranova, Vincent ; Jones, William G. ; Judy D ; Pailes WH ; Schwegler-Berry D
• Description:
  The objective of this study was to compare the cytotoxicity of an asbestiform mineral, crocidolite, to that of its non-asbestiform analogue, riebeckite. Crocidolite had a median fibre length of 11.5 Jim and a surface area of 17 m2 g -1 while the count median diameter of riebeckite was 3 um with a surface area of2.7 m2 g-i. Crocidolite samples contained 20--40 times more particles per mass than riebeckite samples. Cytotoxicity was determined by treating rat alveolar macrophages in culture with various concentrations of particles for 1-3 days and monitoring membrane integrity ,leakage of cytoplasmic and lysosomal enzymes and zymosan-stimulated respiratory burst activity. In general, crocidolite exhibited cytotoxicity at doses as low as 100 ug ml-1. Riebeckite was cytotoxic in most assays at 300 ug ml- 2. Therefore, on a mass concentration basis, crocidolite was more toxic than riebeckite. On an equivalent surface area basis, riebeckite was slightly more toxic than crociodolite. On an equivalent particle count basis, riebeckite was far more toxic. [Description provided by NIOSH]
• Subjects:
  Animals Cytotoxins Minerals Occupational Health Particulate Matter Safety
• Keywords:
  Animal-studies Cytotoxic-effects Cytotoxicity Fibrous-bodies Samplers
• ISBN:
  0080408419
• ISSN:
  0003-4878
• Publisher:
  Pergamon
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  38
• NIOSHTIC Number:
  nn:20025038
• Citation:
  Ann Occup Hyg, Inhaled Particles VII 1994 Jan; 38(Suppl 1):665-673
• Contact Point Address:
  V. Castranova, Division of Respiratory Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
• CAS Registry Number:
  Crocidolite asbestos (CAS RN 12001-28-4)
• Editor(s):
  Dodgson J; McCallum RI
• Federal Fiscal Year:
  1994
• Peer Reviewed:
  True
• Source Full Name:
  Annals of Occupational Hygiene, Inhaled Particles VII
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:247ba9cfd7ca419703cb7724cb87cc632552ce16e448852f19f4c35cf149874cfbb1ce67a28bfc16f5e9e4eaf88b17af1566eb69c59d0939a0ce44ae5e08fac9
• Download URL:
  https://stacks.cdc.gov/view/cdc/196550/cdc_196550_DS1.pdf
• File Type:
  [PDF - 2.21 MB ]