Detection of N-Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) in Cotton Dust: Biological Activities of FMLP Associated with Pulmonary Responses to Cotton Dust Exposure

Details

• Personal Author:
  Castranova, Vincent ; Fedan, Jeffery S. ; Frazer DG ; Ma JKH ; Mo CG
• Description:
  Inhalation of cotton dust (20 mg m - 3 for 6 h) by guinea pigs is marked by infiltration of granulocytes into the airways (11. 7-fold) and airway closure (I.3-fold). In addition, aqueous extracts of cotton dust cause constriction of tracheal smooth muscle in vitro. Analysis of water or acetone extracts of cotton dust by HPLC reveals substantial quantities (mg g-l) of both FMLP and oxidized FMLP. As with cotton dust extracts, FMLP is an agonist of guinea pig tracheal smooth muscle (ECso=91 nM FMLP). Both reduced and oxidized FMLP stimulate chemotaxis and respiratory burst activity in granulocytes. Furthermore, activated pulmonary granulocytes isolated from cotton dust-exposed guinea pigs induce contraction of trachealis muscle in vitro. Thus, FMLP may be involved in the pulmonary response to cotton dust exposure directly by causing airway constriction and indirectly by inducing inflammation characterized by granulocyte infiltration and activation. [Description provided by NIOSH]
• Subjects:
  Animals Dust Laboratories Lung Occupational Health Respiratory System Safety
• Keywords:
  Animal-studies Cotton-dust Dust-analysis Dust-exposure Dust-inhalation In-vitro-study Laboratory-animals Pulmonary-system
• ISBN:
  0080408419
• ISSN:
  0003-4878
• Publisher:
  Pergamon
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  38
• NIOSHTIC Number:
  nn:20025035
• Citation:
  Ann Occup Hyg, Inhaled Particles VII 1994 Jan; 38(Suppl 1):879-885
• Contact Point Address:
  J. S. Fedan, Division of Respiratory Disease Studies, National Institute for Occuaptional Safety and Health, Biochemistry Section, 994 Chestnut Ridge Road, Morgantown, WV, 26505, USA
• Editor(s):
  Dodgson J; McCallum RI
• Federal Fiscal Year:
  1994
• Peer Reviewed:
  True
• Source Full Name:
  Annals of Occupational Hygiene, Inhaled Particles VII
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:71490bdd771c5b79b9014b2f18b8e86442bd42effe05cbb774012485286993de81f564f0a11ae6e944926e70265eb2683022ca9638a30794436219cf5bf1031a
• Download URL:
  https://stacks.cdc.gov/view/cdc/196547/cdc_196547_DS1.pdf
• File Type:
  [PDF - 477.50 KB ]