Detecting Pre-Ovulatory Luteinizing Hormone Surges in Urine

Details

• Personal Author:
  Kesner JS ; Knech EA ; Krieg EF Jr. ; O'Connor JF ; Wilcox AJ
• Description:
  The study objectives were to determine (i) if pre-ovulatory luteinizing hormone (LH) surges, undetected in urine by two immunoradiometric assays (IRMA), were detectable by an ultrasensitive immunofluorometric assay (IFMA) and (ii) the influence of creatinine adjustment on the detection and timing of the urinary LH surges. Daily urine specimens were contributed by healthy 25-36 year old volunteers during 14 ovulatory menstrual cycles for an epidemiological study conducted in 1983-1985. Specimens were selected as having been previously assayed by two IRMA without consistently detecting LH surges. These urine specimens were remeasured using an IFMA and adjusted for creatinine concentration. IFMA measurements revealed unambiguous LH surges in all cycles. Adjusting IRMA urinary LH values for creatinine concentrations revealed previously undetected LH surges in four of eight cycles. Creatinine adjustment also altered the timing of IRMA and IFMA LH surges by 1-5 days. These results demonstrate an IFMA that detects pre-ovulatory LH surges in unpreserved, frozen urine from cycles where such surges were previously undetectable. Further, creatinine adjustment can markedly affect detection and timing of the onset and peak of the urinary LH surge. While our analysis suggests that this adjustment improves the validity of the LH measure, this requires further investigation. [Description provided by NIOSH]
• Subjects:
  Demography Endocrine System Epidemiology Menstruation Disturbances Occupational Health Safety Sex Characteristics Urine Women
• Keywords:
  Author Keywords: Creatinine Demographic-characteristics Hormone-activity Hormones Humans Menstrual-disorders Menstrual Cycle Sex-factors Two-site Immunofluorometric Assay Urinalysis
• ISSN:
  0268-1161
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  New York ; North Carolina ; Ohio ; OSHA Region 2 ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  15-21
• Volume:
  13
• Issue:
  1
• NIOSHTIC Number:
  nn:20024960
• Citation:
  Hum Reprod 1998 Jan; 13(1):15-21
• Contact Point Address:
  Experimental Toxicology Branch, Division of Biomedical and Behavioral Science, National Institute for Occupational Safety and Health, 4676 Columbia Parkway, MS-C23, Cincinnati, OH 45226, USA
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  Human Reproduction
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9d96b741371055edb750bccc131fcb7309c1086885b1190c00c39a749c0635999c4caa5e6d0571d218d51d0270732c2621400efae7054e235e878be7b9867d67
• Download URL:
  https://stacks.cdc.gov/view/cdc/196511/cdc_196511_DS1.pdf
• File Type:
  [PDF - 316.39 KB ]