Inhibition of AP-1 and Neoplastic Transformation by Fresh Apple Peel Extract

Details

• Personal Author:
  Bowman L ; Castranova, Vincent ; Ding M ; Huang C ; Leonard, Stephanie ; Lu Y ; Shi X ; Vallyathan V ; Wang L
• Description:
  Consumption of fruits and vegetables has been associated with a low incidence of cancers and other chronic diseases. Previous studies suggested that fresh apples inhibit tumor cell proliferation. Here we report that oral administration of apple peel extracts decreased the number of nonmalignant and malignant skin tumors per mouse induced by 12-O-tetradecanolyphorbol-13-acetate (TPA) in 7,12-dimethylbenz(a)anthracene-initiated mouse skin. ESR analysis indicated that apple extract strongly scavenged hydroxyl (OH) and superoxide (O(2)(-)) radicals. Mechanistic studies showed that pretreatment with apple peel extract inhibited AP-1 transactivation induced by ultraviolet B irradiation or TPA in JB6 cells and AP-1-luciferase reporter transgenic mice. This inhibitory effect appears to be mediated by the inhibition of ERKs and JNK activity. The results provide the first evidence that an extract from fresh apple peel extract may inhibit tumor promoter-induced carcinogenesis and associated cell signaling, and suggest that the chemopreventive effects of fresh apple may be through its antioxidant properties by blocking reactive oxygen species-mediated AP-1-MAPK activation. [Description provided by NIOSH]
• Subjects:
  Animals Antioxidants Chronic Disease Laboratories Neoplasms Occupational Exposure Occupational Health Safety Skin Skin Diseases
• Keywords:
  Animal-studies Antioxidation Cancer Chronic-degenerative-diseases Chronic-exposure Laboratory-animals Skin-cancer Skin-diseases Skin-tumors
• ISSN:
  0021-9258
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  279
• Issue:
  11
• NIOSHTIC Number:
  nn:20024738
• Citation:
  J Biol Chem 2004 Mar; 279(11):10670-10676
• Email:
  mid5@cdc.gov
• Federal Fiscal Year:
  2004
• NORA Priority Area:
  Research Tools and Approaches: Cancer Research Methods
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Biological Chemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0f3cf250e01150b84b878cfc312a9576106ea524ade17b0daccffc3b60f79f40d1f5239177f661a1bae163618c936ac57d5204d709b66f51ade769cabc6b7736
• Download URL:
  https://stacks.cdc.gov/view/cdc/196376/cdc_196376_DS1.pdf
• File Type:
  [PDF - 368.60 KB ]