Concurrent Fluorescence In Situ Hybridization and Immunocytochemistry for the Detection of Chromosome Aberrations in Exfoliated Bronchial Epithelial Cells

Details

• Personal Author:
  Anderson M ; Belinsky SA ; Crowell RE ; Gilliland FD ; Lechner JF ; Michels R ; Murphy MM ; Neft RE ; Saccomanno G ; Teirney LA ; Timm S
• Description:
  A procedure was developed to allow concurrent detection of chromosome aberrations and identification of bronchial epithelial cells. Fluorescence in situ hybridization for chromosome 7 and immunocytochemistry for cytokeratin were performed on exfoliated bronchial epithelial cells in a sputum sample from a cancer patient. The Spectrum Orange-labeled alpha satellite probe for chromosome 7 produced red fluorescence, nuclei were counterstained with 4,6-diamidino-2-phenylindole (blue), and cytokeratin was visualized using a fluorescein isothiocyanate (FITC)-conjugated secondary antibody (green). This procedure allowed the rapid identification of airway epithelial cells with numerical chromosome aberrations in this sample. Ultimately, this procedure could increase the sensitivity and specificity of sputum cytology as a laboratory diagnostic tool for the early detection of lung cancer. [Description provided by NIOSH]
• Subjects:
  Histocytochemistry Immune System Lung Lung Diseases Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety

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• Keywords:
  Bronchial Cancer Cytochemistry Immunochemistry Lung Cancer Lung Disease Lung Disorders Pulmonary System Disorders Respiratory System Disorders Risk Factors
• ISSN:
  0001-5547
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New Mexico ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  41
• Issue:
  6
• NIOSHTIC Number:
  nn:20024398
• Citation:
  Acta Cytol 1997 Nov-Dec; 41(6):1769-1773
• Contact Point Address:
  Robin E. Neft, Ph.D., Lovelace Respiratory Research Institute, P.O. Box 5890, Alburquerque, NM 87185
• Federal Fiscal Year:
  1998
• Performing Organization:
  University of New Mexico, Department of Medicine, Albuquerque, New Mexico
• Peer Reviewed:
  True
• Start Date:
  19940930
• Source Full Name:
  Acta Cytologica
• End Date:
  19970929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1118bc74899921651ee48726e743a77d43eb53cdf7bebffe163a1b519e9bf3fe50bde268dd5a2d758647194510dc1b9920751b9bd2f90fdc85531b57715a2ae9
• Download URL:
  https://stacks.cdc.gov/view/cdc/196219/cdc_196219_DS1.pdf
• File Type:
  [PDF - 88.82 KB ]