A New Method for the Detection of Neutralizing Antibodies against Mumps Virus

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Matsubara, Keita ; Fujino, Motoko ; Takeuchi, Kaoru ; Iwata, Satoshi ; Nakayama, Tetsuo
• Description:
  Neutralization test is the most reliable method of evaluating immunity against viral diseases but there is no standard procedure for mumps virus, with tests differing in the infectivity of the challenge virus, 50% plaque reduction or complete inhibition of cytopathic effects (CPE), and usage of complement. A reliable, easy, and simple neutralization test for mumps virus was developed in this study. A recombinant mumps virus expressing GFP was generated as a challenge virus. Complement was added to the neutralizing mixture at 1∶200 when stocked serum samples were used. Neutralizing antibody titers were expressed as the reciprocal of the highest dilution that did not exceed two-fold of FU values (GFP expression) of the cell control wells. A total of 1,452 serum samples were assayed by inhibition of GFP expression in comparison with those examined by conventional 100% inhibition of CPE. 1,367 (94.1%) showed similar neutralizing antibody titers when examined by both methods. The GFP expression inhibition assay, using a recombinant mumps virus expressing GFP, is a simple and time- saving method.
• Subjects:
  Adult Animals Antibodies, Neutralizing Antibodies, Viral Cell Line Chlorocebus Aethiops Complement System Proteins Cytopathogenic Effect, Viral Gene Expression Gene Order Genes, Reporter Genetic Vectors Humans Middle Aged Mumps Mumps Virus Neutralization Tests Temperature Vero Cells Viral Plaque Assay Young Adult

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• Source:
  PLoS One. 2013; 8(7).
• Document Type:
  Journal Article
• Volume:
  8
• Issue:
  7
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:43af7440ee8b9fde93bb28680f0a1e747387b3ac116970c768fdb8732188dd31
• Download URL:
  https://stacks.cdc.gov/view/cdc/19598/cdc_19598_DS1.pdf
• File Type:
  [PDF - 511.53 KB ]