Details:

Alternative Title:
PLoS Negl Trop Dis
Personal Author:
López-Gil, Elena ; Lorenzo, Gema ; Hevia, Esther ; Borrego, Belén ; Eiden, Martin ; ... More +
López-Gil, Elena ; Lorenzo, Gema ; Hevia, Esther ; Borrego, Belén ; Eiden, Martin ; Groschup, Martin ; Gilbert, Sarah C. ; Brun, Alejandro Less -
Description:
Background

Rift Valley fever virus (RVFV) is a mosquito-borne pathogen causing an important disease in ruminants often transmitted to humans after epizootic outbreaks in African and Arabian countries. To help combat the spread of the disease, prophylactic measures need to be developed and/or improved.

Methodology/Principal Findings

In this work, we evaluated the immunogenicity and protective efficacy of recombinant plasmid DNA and modified vaccinia virus Ankara (rMVA) vectored vaccines against Rift Valley fever in mice. These recombinant vaccines encoded either of two components of the Rift Valley fever virus: the viral glycoproteins (Gn/Gc) or the nucleoprotein (N). Following lethal challenge with live RVFV, mice immunized with a single dose of the rMVA-Gn/Gc vaccine showed no viraemia or clinical manifestation of disease, but mounted RVFV neutralizing antibodies and glycoprotein specific CD8+ T-cell responses. Neither DNA-Gn/Gc alone nor a heterologous prime-boost immunization schedule (DNA-Gn/Gc followed by rMVAGn/Gc) was better than the single rMVA-Gn/Gc immunization schedule with regards to protective efficacy. However, the rMVA-Gn/Gc vaccine failed to protect IFNAR−/− mice upon lethal RVFV challenge suggesting a role for innate responses in protection against RVFV. Despite induction of high titer antibodies against the RVFV nucleoprotein, the rMVA-N vaccine, whether in homologous or heterologous prime-boost schedules with the corresponding recombinant DNA vaccine, only conferred partial protection to RVFV challenge.

Conclusions/Significance

Given the excellent safety profile of rMVA based vaccines in humans and animals, our data supports further development of rMVA-Gn/Gc as a vaccine strategy that can be used for the prevention of Rift Valley fever in both humans and livestock.


Subject:
[+]
Animals
Antibodies, Neutralizing
Antibodies, Viral
CD8-Positive T-Lymphocytes
Disease Models, Animal
Drug Carriers
Epitopes
Female
Glycoproteins
Mice
Mice, Inbred BALB C
Mice, Knockout
Receptor, Interferon Alpha-beta
Rift Valley Fever
Rift Valley Fever Virus
Survival Analysis
Vaccines, DNA
Vaccines, Synthetic
Vaccinia Virus
Viral Proteins
Viral Vaccines
Viremia

Source:
PLoS Negl Trop Dis. 2013; 7(7).
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:c9f673dbf1c0f4a81ef3a72c7cc23c5b8fb49c37bdc619de4cc1d5c6899adb0f
File Type:

Supporting Files

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