Physiological Role of Tumor Necrosis Factor Alpha in Traumatic Muscle Injury

Details

• Personal Author:
  Hulderman T ; Jensen N ; Luster MI ; McKinstry M ; Mishra M ; Simeonova PP ; Warren GL
• Description:
  Degenerative and regenerative roles of tumor necrosis factor alpha (TNF-alpha), a pro-inflammatory cytokine with pleiotropic functions, were investigated by using TNF receptor 1 and 2 double knockout (TNFR-DKO) and TNF-alpha antibody neutralized mice following traumatic freeze injury to the tibialis anterior muscle. In wild-type control mice, TNF-alpha mRNA transcripts and protein increased following injury and gradually returned to control (uninjured) levels by 13 days. A reduction in MyoD mRNA expression occurred in TNF-alpha-deficient mice, although there were no visible differences in MyoD immunostaining or histological characteristics in regenerating muscles. At 5 days post-injury, the reductions in isometric strength in TNFR-DKO and TNF-alpha-depleted mice did not differ from that of wild-type mice but by 13 days after injury, the TNFR-DKO and TNF-alpha-depleted mice exhibited strength deficits twice that of wild-type mice (i.e., 27-31% vs 13%). Muscle injury was also accompanied by increased expression of interleukin-6 (IL-6), but IL-6-deficient mice demonstrated MyoD expression and recovery of isometric strength similar to that of wild-type mice. These data indicate that TNF-alpha is involved in the recovery of muscle function after traumatic muscle injury, and this effect might be associated with modulation of muscle regulatory genes, including MyoD. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Musculoskeletal System Neoplasms Occupational Health Safety
• Keywords:
  Animal Studies Author Keywords: Muscle Function Inflammation Inflammatory Cytokines Injuries Laboratory Animals Muscle Necrosis Muscles Traumatic Injuries Tumors
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  16
• Issue:
  12
• NIOSHTIC Number:
  nn:20023536
• Citation:
  FASEB J 2002 Oct; 16(12):1630-1632
• Contact Point Address:
  NIOSH 1095 Willowdale Road, Morgantown WV 26505
• Email:
  phs9@cdc.gov
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  True
• Source Full Name:
  FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:266f3d8a473c0669a171c4e7048e0872b72b3c0e72351a0d85a48e84a418eed978e1685432a0e8c1c8c360da387fda2de9bbb728a24335836d392a765f894df9
• Download URL:
  https://stacks.cdc.gov/view/cdc/195906/cdc_195906_DS1.pdf
• File Type:
  [PDF - 818.16 KB ]