Using PCR to Detect Indoor Fungi

Details

• Personal Author:
  Chen BT ; Keswani J ; Ong T ; Zhou G
• Description:
  This research attempts to develop a molecular bioassay that uses a Polymerase Chain Reaction (PCR) technique for detecting fungi commonly found in an indoor environment. During sample preparation, the method of bead-beating was identified as the most effective way for spore breakage and fungal DNA release. In the process of assay development, a fungal primer set, FF2/FRl, was designed and tested with DNA from human, rats, mice, bacteria, pollens and six fungal species. The results based on the crude extracts indicated that the primer set successfully amplified the fungal DNA without any cross-amplification with non-fungal DNA. In addition, higher amplification efficiencies were achieved using 20% nutrient media, rather than water, as the process solution. This PCR assay has a sensitivity of detecting low levels of fungi (2 fungal spores per reaction). [Description provided by NIOSH]
• Subjects:
  Coal Coal Mining Engineering Mining Occupational Health Safety
• Keywords:
  Analytical Methods Analytical Processes Bioassays Fungi Indoor Air Pollution Indoor Environmental Quality Molecular Biology Molecular Structure Sampling Methods
• Document Type:
  Text
• Genre:
  Proceedings ; Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  63-68
• NIOSHTIC Number:
  nn:20023404
• Citation:
  Indoor Air 2002 Jun; :63-68
• Email:
  bdc4@cdc.gov
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Indoor Air 2002, Proceedings: 9th International Conference on Indoor Air Quality and Climate, Montery, California, June 30-July 5, 2002
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c068bc1a80147e2cc58578a84cbff15a4c599ba6206d62a04b9b87ef6743c55f9391d86a3e03acd17d97b195af5014385d1c928cbd19404655007d2f05ecb86f
• Download URL:
  https://stacks.cdc.gov/view/cdc/195851/cdc_195851_DS1.pdf
• File Type:
  [PDF - 155.35 KB ]