Comparison of the Responsiveness of Primary Alveolar Macrophages (AM’s), Primary Alveolar Type II Cells (TII), and a Rat Alveolar Type II Cell Line (RLE-6TN) to Lipopolysaccharide (LPS) and Silica Exposure

Details

• Personal Author:
  Castranova, Vincent ; Kang J ; Kanj R
• Description:
  The goal of this study was to compare the responses of AM's and TII cells' to LPS or silica and eventually determine their mutual interactions. AM's were collected by bronchoalveolar lavage, TII cells were isolated by enzymatic digestion and purified by panning (approximately 20 million cells/rat, 95% pure), and RLE-6TN were commercially obtained. Cells were exposed in vitro to LPS or silica for 18 hours, after which nitric oxide (NO) and tumor necrosis factor-alpha (TNF-a) release were measured in the supernate using a modified Greiss reaction and ELISA, respectively. Cytotoxicity was measured by trypan blue exclusion and lactate dehydrogenase (LDH) activity. Upon LPS exposure, AM's released more NO and TNF-a than TII cells, which in turn were more responsive than RLE-6TN. Peak release of NO and TNF-a occurred at LPS doses of 1-5 ug/ml, 5-10 ug/ml, and 50-100 ug/ml for AM's, TII cells and RLE-6TN cells, respectively. Silica doses below 50 ug/ml failed to stimulate NO or TNF-a production in any of these cell types. These data indicate that LPS is a potent inducer of NO and TNF-a release with AM's being the most responsive and RLE-6TN the least responsive alveolar cell type tested. [Description provided by NIOSH]
• Subjects:
  Aluminum Silicates Cytotoxins Lung Neoplasms Occupational Health Respiratory System Safety Silicon Dioxide
• Keywords:
  Alveolar-cells Cytotoxicity Exposure-levels In-vitro-studies Lung-cells Oxides Silica-dusts Silicates Tumors
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Louisiana ; OSHA Region 3 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  16
• Issue:
  5
• NIOSHTIC Number:
  nn:20023388
• Citation:
  FASEB J 2002 Mar; 16(5):A1146
• Contact Point Address:
  NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal, Experimental Biology 2002, New Orleans, Louisiana, April 20-24, 2002
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f63682a4af6ec406f4cbec6dcc777b36513e611f55d582f7d2f37f624ae2c7901bf0d1f5fecf25a7638e9800276ebc99399d235cdc258d7283e5d9ee64b58c5f
• Download URL:
  https://stacks.cdc.gov/view/cdc/195848/cdc_195848_DS1.pdf
• File Type:
  [PDF - 471.06 KB ]