Enhanced Oxidative Stress in the Skin of Vitamin E Deficient Mice Exposed to Semisynthetic Metal Working Fluids

Details

• Personal Author:
  Castranova, Vincent ; Kisin E ; Kommineni C ; Murray A ; Shvedova AA ; Smith C
• Description:
  Metal working fluids (MWFs) are widely used in industry for metal cutting, drilling, shaping, lubricating, and milling. Many occupational health concerns have arisen for workers exposed to MWFs. It has been reported earlier that occupational exposure to MWFs causes allergic and irritant contact dermatitis. Previously, we have shown that dermal exposure of female and male B6C3F1 mice to 5% MWFs for 3 months resulted in accumulation of mast cells and elevation of histamine in the skin. Topical exposure to MWFs also resulted in elevated oxidative stress in the liver of both sexes and the testes in males. The goal of this study was to evaluate whether preexisting oxidative stress in the skin exacerbated mast cell influx after MWFs treatment. Oxidative stress in the skin of B6C3F1 mice was generated by dietary vitamin E deprivation. Mice were given vitamin E deficient (5-10 i.v./kg of vitamin E) or basal (50 i.v./kg of vitamin E) diets for 34 weeks. Topical treatment with MWFs (100 microl, 30%) started after 18 weeks of alimentary vitamin E deprivation. Histology of the skin after 16 weeks of exposure to MWFs revealed a 53% increase in mast cell accumulation in vitamin E deficient diets compared to mice given a vitamin E sufficient diet. Total antioxidant reserve in skin of vitamin E deprived mice treated with MWFs was decreased by 66% as compared to those mice given a vitamin E sufficient diet. GSH and protein thiols in the dermis of vitamin E deprived mice exposed to MWFs were also decreased 39 and 42%, respectively, as compared to mice given basal diet. This study clearly delineates the role of oxidative stress in enhancing mast cell accumulation caused by topical exposure to MWFs. [Description provided by NIOSH]
• Subjects:
  Animals Antioxidants Burns Dermatitis Laboratories Manufacturing And Industrial Facilities Neoplasms Occupational Health Safety Skin Skin Diseases

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• Keywords:
  Animal Studies Author Keywords: Metal Working Fluids Cancer Exposure Limits GSH Inflammation In Vitro Studies Laboratory Animals Machine Shop Workers Metal Industry Workers Oxidative Stress Skin Skin Diseases Skin Exposure Vitamin E

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• ISSN:
  0300-483X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  135-143
• Volume:
  176
• Issue:
  1
• NIOSHTIC Number:
  nn:20023342
• Citation:
  Toxicology 2002 Jul; 176(1-2):135-143
• Contact Point Address:
  Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Mail Stop 2015, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505
• CAS Registry Number:
  Chloroform (CAS RN 67-66-3) ; Chromium (CAS RN 7440-47-3) ; Hexane (CAS RN 110-54-3) ; Methanol (CAS RN 67-56-1) ; Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Disease and Injury: Allergic and Irritant Dermatitis
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0552e08037f53f5e8b58eb46a8321d765d6c3e7bf85ba6bbbcd0120970480cc83fd8c235267ba9e9d8248541f7fe63c45e4fe9a883bc3a62a4d1772071df3206
• Download URL:
  https://stacks.cdc.gov/view/cdc/195840/cdc_195840_DS1.pdf
• File Type:
  [PDF - 323.01 KB ]