Age-dependent differential gene expression in exposure response of contraction-induced muscle injury

Details

• Personal Author:
  Baker BA ; Cutlip, Robert G. ; Ensey J ; Hollander MS ; Kashon ML ; Li S ; Baker BA ; Cutlip, Robert G. ; Ensey J ; Hollander MS ; Kashon ML ; Li S
• Description:
  A high volume of repetitions that results in contraction-induced muscle injury in young rats has been shown to have a more robust genomic response to the physiological trauma than similar paradigms in old rats. Thus, the purpose was to identify if this age-dependent genomic signature was present in skeletal muscle following lower number of muscular contractions. The left dorsiflexor muscles of young (3 mo) and old (30 mo) male Fischer 344 x BN rats were injured using 30, 80 and 150 stretch-shortening contractions (SSCs) at a velocity of 500 degrees/s in vivo. Seventy-two hours following exposure the left tibialis anterior muscle was harvested and RNA was isolated. cRNA samples were prepared and loaded onto Sentrix Rat-Ref12 Beadchips for gene array analyses. With respect to contraction number, there was a graded up-regulation in response of genes related to the inflammatory response (i.e. SPP1, MMP12, CD68), cell death (CTSS, CTSB, CTSL1) and muscle regeneration (i.e. ANXA1,S100A11, RPS6) in young rats following injurious SSCs, yet the same genes have an attenuated, and even opposite, response in old rats regardless of contraction number. These data suggest desensitization in the old rats' ability to mount a competent response to graded contraction-induced injury stimuli, which, ultimately, may adversely affect recovery kinetics following injury with increased age. [Description provided by NIOSH]
• Subjects:
  Age Factors Animals Genetics Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Age-factors Analytical-processes Cell-biology Cell-function Cellular-function Cellular-reactions Genes Laboratory-animals Muscle-physiology Muscles Muscular-disorders Musculoskeletal-system Musculoskeletal-system-disorders

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• NIOSHTIC Number:
  nn:20042863
• Citation:
  FASEB J 2013 Apr; 27(Meeting Abstracts):1212.9
• Federal Fiscal Year:
  2013
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal
• Supplement:
  Meeting Abstracts
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fabca7973e63229ab0d22a9c97b6cd92faa9a3480338f22d9883475a89549c9fbeaa30e0622cbe2e13ab951727009f431833cf5b5553e9bc58f5f35b37c6390d
• Download URL:
  https://stacks.cdc.gov/view/cdc/194947/cdc_194947_DS1.pdf
• File Type:
  [PDF - 28.73 KB ]