Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways

Details

• Personal Author:
  Bowman L ; Castranova, Vincent ; Ding M ; Leonard, Stephen S. ; Magaye R ; Zhao J
• Description:
  In this study, apoptosis and related signaling induced by WC-Co nanoparticles were investigated in JB6 cells and rat lung macrophages. Electron spin resonance (ESR) and fluorescent staining indicated that both WC-Co nanoparticles and fine particles stimulated reactive oxygen species (ROS) generation. Catalase exhibited an inhibitory effect on WC-Co nanoparticle-induced ROS as well as mitochondrial membrane permeability damage. Further study indicated that WC-Co nanoparticles elicited higher cytotoxicity and apoptotic induction than fine particles. Western blot analysis showed activation of proapoptotic factors including Fas, Fas-associated protein with death domain (FADD), caspase 3, 8 and 9, BID and BAX. In addition, both cytochrome c and apoptosis-inducing factor (AIF) were upregulated and released from mitochondria to the cytoplasm. Our findings demonstrate that, on a mass basis, WC-Co nanoparticles exhibit higher cytotoxicity and apoptotic induction than fine particles. Apoptosis induced by WC-Co nanoparticles and fine particles involves both extrinsic and intrinsic apoptosis pathways. [Description provided by NIOSH]
• Subjects:
  Animals Cytotoxins Laboratories Lung Occupational Health Particulate Matter Respiratory System Safety
• Keywords:
  Apoptosis-inducing Factor Author Keywords: JB6 Cells Cytochrome C Cytotoxicity Laboratory-animals Mitochondria Nanotechnology Particulates Rat Lung Macrophages
• ISSN:
  1019-6439
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  42
• Issue:
  4
• NIOSHTIC Number:
  nn:20042450
• Citation:
  Int J Oncol 2013 Apr; :42(4):1349-1359
• Contact Point Address:
  Jinshun Zhao, Ningbo Univ, Zhejiang Prov Key Lab Pathol & Physiol Technol, Sch Med, Dept Prevent Med, 818 Fengua Rd, Ningbo 315211, Zhejiang, Peoples R China
• Email:
  zhaojinshun@nbu.edu.cn
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  International Journal of Oncology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0306d4c02360472da26a681b7d0844ea13752f64e92da4deb2ac139b463bc2bdbac79606f5c41486dadfd2eadb8775164995aa657f2e4541032d21603473b767
• Download URL:
  https://stacks.cdc.gov/view/cdc/194673/cdc_194673_DS1.pdf
• File Type:
  [PDF - 7.25 MB ]