Macrophage toxicity in response to particles collected from indium-tin oxide production

Details

• Personal Author:
  Badding MA ; Castranova, Vincent ; Cummings, Kristin J. ; Dunnick KM ; Fix NR ; Leonard, Stephen S.
• Description:
  Occupational exposure to indium compounds has recently been associated with lung disease among workers in the indium-tin oxide (ITO) industry. Previous studies have suggested that autoantibodies and reactive oxygen species (ROS) may play a role in the development of pulmonary lesions following indium compound exposures. However, the molecular mechanisms behind indium compounds' toxicity remain largely unknown. Thus, we aim to uncover how compounds encountered in the ITO production process affect cultured macrophages and ultimately, contribute to the pathogenesis of indium lung disease. The indium compounds used in this study were collected from different process stages at an ITO production facility. Darkfield microscopy has revealed that various indium compounds interact with the cells as early as 5 minutes post-exposure, suggesting that cellular reactions to the ITO compounds may be occurring very rapidly. Indeed, using electron spin resonance (ESR) to measure ROS generation, we found that various collected indium compounds produce free radicals in the presence and absence of RAW 264.7 mouse macrophages within 5 minutes. We also hypothesize that indium compound particle uptake by macrophages leads to subsequent cellular damage, which could be contributing to lung pathology. Therefore, various imaging techniques are being used to observe particle association with and uptake by macrophages over a time course. Current studies are underway to evaluate the effects of the collected indium compounds on intracellular ROS production, lipid peroxidation, DNA damage, and cell proliferation over the same time course. These findings will provide a foundation for the molecular basis behind an emerging occupational health issue and assist in the prevention indium lung disease. [Description provided by NIOSH]
• Subjects:
  Antibodies Cells, Cultured DNA Free Radicals Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety Toxicology

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• Keywords:
  Antibody-response Cell-alteration Cell-cultures Cellular-function Cellular-reactions Cellular-uptake DNA-damage Dose-response Exposure-levels Free-radical-generation Free-radicals Laboratory-animals Lipid-peroxidation Lung-cells Lung-disorders Lung-function Lung-irritants Oxides Pathogenesis Pulmonary-function Pulmonary-system-disorders Respiratory-system-disorders Tin-oxides

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; OSHA Region 6 ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  132
• Issue:
  1
• NIOSHTIC Number:
  nn:20042442
• Citation:
  Toxicologist 2013 Mar; 132(1):427
• CAS Registry Number:
  Indium (CAS RN 7440-74-6) ; Indium tin oxide (In1.69Sn0.15O2.85) (CAS RN 71243-84-0)
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 52nd Annual Meeting and ToxExpo, March 10-14, 2013, San Antonio, Texas
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:580dc7bfeb969036a73cd74aee7a28b54312aecc5ac8ace0bb95d5a5b61dbdaab70c2e8e7bead593af26bf4f328d3501e14aaf636cc2ed4c06ba7b574d805525
• Download URL:
  https://stacks.cdc.gov/view/cdc/194665/cdc_194665_DS1.pdf
• File Type:
  [PDF - 582.75 KB ]