A multi-center ring trial of allergen analysis using fluorescent multiplex array technology

Details

• Personal Author:
  Arbes SJ Jr. ; Calatroni A ; Chapman MD ; Filep S ; Hamilton, Robert G. ; Heederik DJJ ; King EM ; Krop EJM ; Metwali N ; Milton D ; Mitchell HE ; Platts-Mills T ; Schmechel D ; Sever ML ; Smith B ; Sordillo JE ; Thorne PS ; van Ree R ; Zeldin DC
• Description:
  Background: Consistent performance of allergen assays is essential to ensure reproducibility of exposure assessments for investigations of asthma and occupational allergic disease. This study evaluated intra- and inter-laboratory reproducibility of a fluorescent multiplex array, which simultaneously measures eight indoor allergens in a single reaction well. Methods: A multi-center study was performed in nine laboratories in the US and Europe to determine the inter-laboratory variability of an 8-plex array for dust mite, cat, dog, rat, mouse and cockroach allergens. Aliquots of 151 dust extract samples were sent to participating centers and analyzed by each laboratory on three separate occasions. Agreement within and between laboratories was calculated by the concordance correlation coefficient (CCC). Results: Results were obtained for over 32,000 individual allergen measurements. Levels covered a wide range for all allergens from below the lower limit of detection (LLOD = 0.1-9.8 ng/ml) to higher than 6800 ng/ml for all allergens except Mus m 1, which was up to 1700 ng/ml. Results were reproducible within as well as between laboratories. Within laboratories, 94% of CCC were = 0.90, and 80% of intra-laboratory results fell within a 10% coefficient of variance (CV%). Results between laboratories also showed highly significant positive correlations for all allergens (approximately 0.95, p < 0.001). Overall means of results were comparable, and inter-laboratory CV% for all allergens except Rat n 1 ranged between 17.6% and 26.6%. Conclusion: The data indicate that performance criteria for fluorescent multiplex array technology are reproducible within and between laboratories. Multiplex technology provides standardized and consistent allergen measurements that will streamline environmental exposure assessments in allergic disease. [Description provided by NIOSH]
• Subjects:
  Air Pollution, Indoor Asthma Asthma, Occupational Dust Environmental Monitoring Hypersensitivity Laboratories Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety Urogenital System

  More +
• Keywords:
  Allergens Analytical-processes Author Keywords: Allergen Measurement Diseases Dusts Exposure-assessment Immunoassay Indoor Air Quality Multiplex Array Occupational Health Pulmonary-system Pulmonary-system-disorders Reproduction Sampling

  More +
• ISSN:
  0022-1759
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Iowa ; Maryland ; Massachusetts ; North Carolina ; OSHA Region 1 ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 7 ; Virginia ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  89-95
• Volume:
  387
• Issue:
  1
• NIOSHTIC Number:
  nn:20042384
• Citation:
  J Immunol Methods 2013 Jan; 387(1-2):89-95
• Contact Point Address:
  Eva King, INDOOR Biotechnologies Inc., 1216 Harris Street, Charlottesville, VA 22903
• Email:
  eking@inbio.com
• Federal Fiscal Year:
  2013
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Immunological Methods
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d8a7494e220a6f53edc2ce14814ad7489d220280e12e1e0bf1fbb6e790f5c745a73934fed9f776b5667936808bd12922c8a9da7386976ff349a86146169dc3c6
• Download URL:
  https://stacks.cdc.gov/view/cdc/194617/cdc_194617_DS1.pdf
• File Type:
  [PDF - 1001.36 KB ]