Mapping of phospholipids by MALDI imaging (MALDI-MSI): realities and expectations

Details

• Personal Author:
  Amoscato AA ; Bayir H ; Dixon CE ; Kagan VE ; Kochanek PM ; Long JB ; Pitt BR ; Sparvero LJ
• Description:
  Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has emerged as a novel powerful MS methodology that has the ability to generate both molecular and spatial information within a tissue section. Application of this technology as a new type of biochemical lipid microscopy may lead to new discoveries of the lipid metabolism and biomarkers associated with area-specific alterations or damage under stress/disease conditions such as traumatic brain injury or acute lung injury, among others. However there are limitations in the range of what it can detect as compared with liquid chromatography-MS (LC-MS) of a lipid extract from a tissue section. The goal of the current work was to critically consider remarkable new opportunities along with the limitations and approaches for further improvements of MALDI-MSI. Based on our experimental data and assessments, improvements of the spectral and spatial resolution, sensitivity and specificity towards low abundance species of lipids are proposed. This is followed by a review of the current literature, including methodologies that other laboratories have used to overcome these challenges. [Description provided by NIOSH]
• Subjects:
  Biochemistry Biomarkers Chemistry Chemistry, Analytic Energy Metabolism Laboratories Lipids Lung Lung Diseases Mass Spectrometry Nervous System Nervous System Diseases Occupational Health Respiratory System Safety

  More +
• Keywords:
  Analytical-chemistry Analytical-instruments Analytical-methods Analytical-processes Brain-damage Brain-disorders Brain-function Chemical-analysis Chemical-indicators Chemical-structure Drug-interaction Drugs Laboratory-techniques Lasers Lung-disorders Lung-function Mass-spectrometry Metabolism Microscopy Molecular-biology Molecular-structure Pharmacology Phospholipids Physical-chemistry Tissue-culture

  More +
• ISSN:
  0009-3084
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  165
• Issue:
  5
• NIOSHTIC Number:
  nn:20042135
• Citation:
  Chem Phys Lipids 2012 Jul; 165(5):545-562
• Contact Point Address:
  Louis J. Sparvero, Center for Free Radical and Antioxidant Health, Department of Environmental and Occupational Health, University of Pittsburgh, Bridgeside Point, 100 Technology Drive, Suite 350, Pittsburgh, PA 15219-3130, USA
• Email:
  ljs39@pitt.edu
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  False
• Start Date:
  20050701
• Source Full Name:
  Chemistry and Physics of Lipids
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f6ecdb88296841efeb39a72f642cee3cf59bd1bc9c9e50f0a8734909a80d2133360b366aa3c76e468ebb0680338388891f6e813949579bca4b9b25927a2732b6
• Download URL:
  https://stacks.cdc.gov/view/cdc/194456/cdc_194456_DS1.pdf
• File Type:
  [PDF - 4.35 MB ]