Toxicological analysis of limonene reaction products using an in vitro exposure system

Details

• Personal Author:
  Anderson, Stacey E. ; Khurshid SS ; Lukomska E ; Mead BJ ; Wells JR
• Description:
  Epidemiological investigations suggest a link between exposure to indoor air chemicals and adverse health effects. Consumer products contain reactive chemicals which can form secondary pollutants which may contribute to these effects. The reaction of limonene and ozone is a well characterized example of this type of indoor air chemistry. The studies described here characterize an in vitro model using an epithelial cell line (A549) or differentiated epithelial tissue (MucilAir). The model is used to investigate adverse effects following exposure to combinations of limonene and ozone. In A549 cells, exposure to both the parent compounds and reaction products resulted in alterations in inflammatory cytokine production. A one hour exposure to limonene + ozone resulted in decreased proliferation when compared to cells exposed to limonene alone. Repeated dose exposures of limonene or limonene + ozone were conducted on MucilAir tissue. No change in proliferation was observed but increases in cytokine production were observed for both the parent compounds and reaction products. Factors such as exposure duration, chemical concentration, and sampling time point were identified to influence result outcome. These findings suggest that exposure to reaction products may produce more severe effects compared to the parent compound. [Description provided by NIOSH]
• Subjects:
  Air Pollution, Indoor Chemistry Environmental Health Epidemiology Occupational Health Ozone Safety
• Keywords:
  Author Keywords: Indoor Air Cell-function Cellular-function Chemical-composition Chemical-properties Exposure-levels Indoor-air-pollution Indoor-environmental-quality Limonene Pollutants Secondary Oxidation Products Tissue-culture

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• ISSN:
  0887-2333
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 6 ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  27
• Issue:
  2
• NIOSHTIC Number:
  nn:20041937
• Citation:
  Toxicol In Vitro 2013 Mar; 27(2):721-730
• Contact Point Address:
  Stacey E. Anderson, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505
• Email:
  sanderson4@cdc.gov
• CAS Registry Number:
  Limonene (CAS RN 138-86-3) ; Ozone (CAS RN 10028-15-6)
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology in Vitro
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:78be35dc1b9221b1ffd7f616c12b7c62a240960ad17a36d01244137b832aeee33bfdb82b84e4a6f9718cdc5546a28395fa89322f290d6129d5ba6503bfbfcb15
• Download URL:
  https://stacks.cdc.gov/view/cdc/194344/cdc_194344_DS1.pdf
• File Type:
  [PDF - 494.14 KB ]