Cochlear base-apex differences in cell-death pathways following exposure to low-frequency noise

Details

• Personal Author:
  Bohne BA ; Harding GW ; Lee SC
• Description:
  Objective: To determine what death pathways are followed by noise-damaged outer hair cells (OHCs). Methods: Chinchillas were exposed to a 0.5-kHz octave band of noise for 24 h. Administration of trypan blue in vivo was used to stain nuclei in cells with leaky plasma membranes. After removing unbound dye, the cochleae were fixed, plasticembedded then dissected to obtain flat preparations of the entire organ of Corti (OC). Using morphological criteria, dying and injured OHCs were classified as following an apoptotic or oncotic death pathway, or a pathway that was neither apoptotic nor oncotic. Results: The exposure caused scattered loss of OHCs in the apical OC and, sometimes, focal hair-cell losses in the basal OC. In the apical OC, many dying OHCs appeared to be following a death pathway that was different from apoptosis and oncosis. In the basal OC, oncotic OHCs were found at the edges of focal lesions. Conclusion: The morphological appearance of dying OHCs in the apical OC was distinct from those following an apoptotic or oncotic death pathway, suggesting that these OHCs were following a previously unidentified death pathway. [Description provided by NIOSH]
• Subjects:
  Animals Hearing Loss, Noise-Induced Laboratories Noise Occupational Health Safety Sense Organs
• Keywords:
  Audiological-testing Author Keywords: Cell-death Pathways Cell-damage Chinchilla Exposure-levels Hazards Histopathology Laboratory-animals Noise-exposure Noise-induced-hearing-loss Noise-levels Noise-measurement Noise Damage

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• ISSN:
  1874-4281
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Missouri ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  29-43
• Volume:
  2
• Issue:
  1
• NIOSHTIC Number:
  nn:20041559
• Citation:
  Open Otorhinolaryngol J 2008 Feb; 2(1):29-43
• Contact Point Address:
  Barbara A. Bohne, Department of Otolaryngology - Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO 63110
• Email:
  bohneb@ent.wustl.edu
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Manufacturing ; Wholesale and Retail Trade
• Performing Organization:
  Washington University, St. Louis
• Peer Reviewed:
  True
• Start Date:
  20000401
• Source Full Name:
  The Open Otorhinolaryngology Journal
• End Date:
  20130914
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5c57e94be57ff91ebee9c7bde354c2b5341076a6a5f7ad2ade91dc83a60729d28848333e8e4cd4c22d9bdca51c6b18e347974f0e49534688899b3e60ccd6995a
• Download URL:
  https://stacks.cdc.gov/view/cdc/194094/cdc_194094_DS1.pdf
• File Type:
  [PDF - 3.90 MB ]