Quantitative determination of total aliphatic isocyanates on skin and surfaces using the map reagent

Details

• Personal Author:
  Bello D ; Liu Y ; Streicher R ; Woskie S ; Youngs F
• Description:
  There is increasing interest in the effects of dermal exposure to isocyanates. One of the limitations with determining dermal exposures has been the lack of a suitable analytical method for the quantification of isocyanates sampled from skin and work surfaces. Sample preparation and analysis were performed according to NIOSH draft Method 5525 for total aliphatic isocyanates in air with some modifications. The pads, impregnated with polypropylene glycol, were obtained from a commercial source. Once a surface or area of skin has been wiped, the pad is placed in the field in a solution of 1-(9-anthracenylmethyl) piperazine (MAP) in methylene chloride for immediate extraction and reaction. Samples are subsequently acetylated to react with excess MAP reagent then processed through a 0.45um filter and a reversed-phase, solid phase extraction (SPE) cartridge. The SPE cartridge significantly reduces interferences from surface and skin wipe samples. The compounds of interest are eluted from the SPE cartridge with acetonitrile and methanol. The analytical method LOD has been conservatively estimated to be 10 ng for both HDI and IPDI. Laboratory spiking of pads was performed to determine extraction efficiency. Pads were spiked with oligomers of HDI isocyanurate (n=24) and IPDI (n=21) at seven different concentrations, ranging from 0.067 to 3.1 and 0.041 to 1.1 ug NCO, respectively. Recoveries for HDI and IPDI were found to be concentration dependent. Correction factors for the recovery of HDI and IPDI were determined from the regression equations of spiked versus recovered oligomer. The HDI oligomer showed an average recovery of 79% while the IPDI oligomer showed an average recovery of 66%. The skin and surface wipe method described here is shown to be a sensitive and reliable approach for the quantification of total aliphatic isocyanates. [Description provided by NIOSH]
• Subjects:
  Chemistry Chemistry, Analytic Fatty Acids Isocyanates Occupational Health Safety Skin Workplace
• Keywords:
  Aliphatic-compounds Analytical-chemistry Analytical-methods Analytical-processes Sampling-methods Skin-exposure Work-areas
• Publisher:
  American Industrial Hygiene Association
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Connecticut ; Massachusetts ; Ohio ; OSHA Region 1 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  70-71
• NIOSHTIC Number:
  nn:20041441
• Citation:
  American Industrial Hygiene Conference and Exposition, June 2-7, 2001, New Orleans, Louisiana. Fairfax, VA: American Industrial Hygiene Association, 2001 Jun; :70-71
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  False
• Source Full Name:
  American Industrial Hygiene Conference and Exposition, June 2-7, 2001, New Orleans, Louisiana
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c99ca61b693a7c80740a8a04de874031694dfa1371c0e87c630494a6fe02bbc53ec53b6c92eb629c2be19b54b78d340739486c411ba4e4b7d17a73b20265a4ab
• Download URL:
  https://stacks.cdc.gov/view/cdc/194012/cdc_194012_DS1.pdf
• File Type:
  [PDF - 241.02 KB ]