Assessment of pulmonary fibrogenic potential of multiwalled carbon nanotubes in human lung cells

Details

• Personal Author:
  Castranova, Vincent ; Chen BT ; Mercer RR ; Mishra A ; Rojanasakul Y ; Wang L
• Description:
  Multiwalled carbon nanotubes have been shown to possess unusual fibrogenic activity in vivo and are currently the focus of intense toxicological investigations. This study further determines the fibrogenic potential of well-dispersed MWCNT in human lung cell culture models and to develop a novel platform for understanding the cellular mechanisms of MWCNT-induced lung fibrosis. Survanta, a natural lung surfactant, showed effectiveness in dispersing agglomerates of MWCNT to fine structures similar in size to aerosolized one. At relevant low doses (0.002-0.2 microgram/cm2), MWCNT exhibited a dose-dependent bio-effect on the human lung epithelial cells which is more pronounced in dispersed-MWCNT compared to non-dispersed form. Significantly elevated levels of fibrogenic mediators, such as transforming growth factor-â1 and matrix metalloprotienases-9 were observed in the dispersed-MWCNT treated lung epithelial cells. Based on previous in vivo studies showing that dispersed-MWCNT penetrated the interstitium and caused rapid interstitial fibrosis, we evaluated the potential direct interaction between lung fibroblasts and MWCNT. Direct stimulation of human lung fibroblast cell proliferation, collagen expression and fibroblast growth factor-2 were observed which suggests novel mechanisms of MWCNT-induced lung fibrosis. Our results indicate that the dispersion status of MWCNT determines their fibrogenic activity which is consistent with in vivo findings. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Fibrosis Lung Occupational Health Respiratory System Safety Surface-Active Agents Toxicology
• Keywords:
  Cell-cultures Cell-function Cellular-function Fibrogenesis Fibrogenicity Humans Lung-fibrosis Nanotechnology Surfactants
• ISSN:
  1687-4110
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  2012
• NIOSHTIC Number:
  nn:20040850
• Citation:
  J Nanomater 2012 Mar; 2012:930931
• Contact Point Address:
  AnuragMishra, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505
• Email:
  amishra@hsc.wvu.edu
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Nanomaterials
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:dca77bbf1e80175d9d7acba91656b929b95fc25ecbb30a19bf36cb3dd32ed558e6ae51340153b267cbf23d118acb2759758297c0d6e34369de392cfb135b9c86
• Download URL:
  https://stacks.cdc.gov/view/cdc/193569/cdc_193569_DS1.pdf
• File Type:
  [PDF - 3.72 MB ]