Adsorption of surfactant lipids by single-walled carbon nanotubes in mouse lung upon pharyngeal aspiration

Details

• Personal Author:
  Amoscato AA ; Balasubramanian K ; Fadeel B ; Feng WH ; Gou P ; Kagan VE ; Kapralov AA ; Kisin ER ; Klein-Seetharaman J ; Kotchey GP ; Mallampalli RK ; Ray P ; Shvedova AA ; Sparvero LJ ; Star A ; Winnica DE ; Yanamala N
• Description:
  The pulmonary route represents one of the most important portals of entry for nanoparticles into the body. However, the in vivo interactions of nanoparticles with biomolecules of the lung have not been sufficiently studied. Here, using an established mouse model of pharyngeal aspiration of single-walled carbon nanotubes (SWCNTs), we recovered SWCNTs from the bronchoalveolar lavage fluid (BALf), purified them from possible contamination with lung cells, and examined the composition of phospholipids adsorbed on SWCNTs by liquid chromatography mass spectrometry (LC-MS) analysis. We found that SWCNTs selectively adsorbed two types of the most abundant surfactant phospholipids: phosphatidylcholines (PC) and phosphatidylglycerols (PG). Molecular speciation of these phospholipids was also consistent with pulmonary surfactant. Quantitation of adsorbed lipids by LC-MS along with the structural assessments of phospholipid binding by atomic force microscopy and molecular modeling indicated that the phospholipids (approximately 108 molecules per SWCNT) formed an uninterrupted "coating" whereby the hydrophobic alkyl chains of the phospholipids were adsorbed onto the SWCNT with the polar head groups pointed away from the SWCNT into the aqueous phase. In addition, the presence of surfactant proteins A, B, and D on SWCNTs was determined by LC-MS. Finally, we demonstrated that the presence of this surfactant coating markedly enhanced the in vitro uptake of SWCNTs by macrophages. Taken together, this is the first demonstration of the in vivo adsorption of the surfactant lipids and proteins on SWCNTs in a physiologically relevant animal model. [Description provided by NIOSH]
• Subjects:
  Biochemistry Laboratories Lipids Lung Mass Spectrometry Occupational Health Respiratory System Safety Surface-Active Agents
• Keywords:
  Alkylamines Alveolar-cells Analytical-models Analytical-processes Atomic-absorption-spectroscopy Author Keywords: Carbon Nanotubes Biochemical-analysis Coatings Hydrophobic-bonds In-vivo-study Laboratory-animals Laboratory-testing Liquid-chromatography Lung-cells Lung-function Macrophages Mass-spectrometry Molecular-biology Molecular-structure Nanotechnology Phospholipids Proteins Pulmonary-function Pulmonary-system Surfactant Surfactants

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• ISSN:
  1936-0851
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  6
• Issue:
  5
• NIOSHTIC Number:
  nn:20040811
• Citation:
  ACS Nano 2012 May; 6(5):4147-4156
• Contact Point Address:
  Valerian E. Kagan, Department of Environmental and Occupational Health, University of Pittsburgh, Bridgeside Point, 100 Technology Drive, Room 330, BRIDG, Pittsburgh, PA 15219-3130, USA
• Email:
  kagan@pitt.edu
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  ACS Nano
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1e343d71f3bd6811e74ed5e19f56100cc7c5a0b146ef5b6569368397831c2a1465efa9097fbe73af54672387ad73888e6f36bb384f28520240029efa2f146810
• Download URL:
  https://stacks.cdc.gov/view/cdc/193542/cdc_193542_DS1.pdf
• File Type:
  [PDF - 555.17 KB ]