Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice

Details

• Personal Author:
  Allen BL ; Conroy J ; Fadeel B ; Feng WH ; Kagan VE ; Kapralov AA ; Kisin ER ; Konduru NV ; Kotchey GP ; Lang MA ; Mead AD ; Mercer RR ; Mohamed BM ; Murray AR ; Shvedova AA ; St. Croix CM ; Star A ; Volkov Y ; Watkins SC
• Description:
  Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and proinflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations. [Description provided by NIOSH]
• Subjects:
  Animals Carcinogens Cells, Cultured Fibrosis Laboratories Lung Occupational Health Respiratory Function Tests Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Animal-studies Cell-biology Cell-cultures Cell-function Laboratory-animals Mesothelial-cells Nanotechnology Oxidative-processes Pulmonary-function Pulmonary-function-tests Pulmonary-system Pulmonary-system-disorders Stress

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• ISSN:
  1932-6203
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  7
• Issue:
  3
• NIOSHTIC Number:
  nn:20040592
• Citation:
  PLoS One 2012 Mar; 7(3):e30923
• Email:
  kagan@pitt.edu
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Mining ; Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  PLoS One
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4d40191bb1d26ec4c9cc4bcd99ebb2f17290da3b3c025f1d5994c5bab1dc1bc1438504c88f7ac25b5b5f894c519f142e7273e75b2d7aa6b0cbd3b0395330194c
• Download URL:
  https://stacks.cdc.gov/view/cdc/193393/cdc_193393_DS1.pdf
• File Type:
  [PDF - 521.79 KB ]