Effects of carbon-based nanomaterials on primary human immune-competent cells

Details

• Personal Author:
  Fadeel B ; Kisin E ; Murray A ; Shvedova A ; Torres Andon F ; Xiao L
• Description:
  Due to their novel electrical, optical, mechanical and chemical properties, carbon-based nanomaterials are currently of great interest for a variety of technological as well as biomedical applications. In this study, we investigated the interaction of three carbon-based nanomaterials with immune-competent cells namely graphene oxide (GO), a 2-D nanomaterial composed of layers of carbon atoms forming six-member rings; single-walled carbon nanotubes (SWCNT), a 1-D nanomaterial formed by the rolling of graphene sheets into hollow tubes, and 3-D hollow carbon spheres (HCS). The materials were first confirmed to be free from endotoxin contamination. We then compared the effect of these nanomaterials on cell viability by Trypan Blue exclusion using primary human monocyte-derived macrophages (HMDM). No significant loss of cell viability was seen in cells treated with SWCNTs or GO up to 100 microg/ml up to 48 h, while a dose-dependent cytotoxic effect was seen for HCS. Cellular uptake of the three nanomaterials was monitored by transmission electron microscopy. We also studied the production of the pro-inflammatory cytokines interleukin (IL)-1beta and IL-1alpha, using LPS-primed HMDM. Dose- and time-dependent activation of IL-1beta was noted for cells incubated with SWCNT and HCS, while GO only induce the secretion of IL-1beta, but to a lesser degree, at the highest dose (100 microg/ml). This work reveals immunotoxicity and/or immunostimulatory effects of three carbon-based nanomaterials. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Hazardous Substances Immune System Lung Microbiology Occupational Health Particulate Matter Respiratory System Safety
• Keywords:
  Biohazards Biomedical-engineering Cell-function Cellular-uptake Cytotoxic-effects Dose-response Health-hazards Immune-reaction Immune-system Immunotoxins Lung-cells Microscopic-analysis Microscopy Nanotechnology Oxidative-processes Oxides Particulates Pulmonary-system Toxic-effects Toxic-materials

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  126
• NIOSHTIC Number:
  nn:20040566
• Citation:
  Toxicologist 2012 Mar; 126(Suppl 1):274
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Mining
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 51st Annual Meeting and ToxExpo, March 11-15, 2012, San Francisco, California
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5682a77811386d72701523efc3034ad4df1903b59386ec74da2a7c3425cfd6703e294345bcced1cacd5b36db339c6a0c4680277282a82e491e1d739a62956ea6
• Download URL:
  https://stacks.cdc.gov/view/cdc/193370/cdc_193370_DS1.pdf
• File Type:
  [PDF - 474.48 KB ]