Assessment of pulmonary toxicity of functionalized multiwall carbon nanotubes in vitro

Details

• Personal Author:
  Castranova, Vincent ; Derk R ; Mishra A ; Rojanasakul Y ; Schwegler-Berry D ; Stueckle, Todd A. ; Wang L ; Wu N
• Description:
  Multi-walled Carbon nanotubes (MWCNT), with their unique physico-chemical properties such as diameter less than 100 nm and a large surface area, have a number of current and proposed applications in health care and consumer products. MWCNT can also be toxic due to fiber shape and large surface area. Previous studies have reported that MWCNT exposure in mice caused rapid and progressive interstitial lung fibrosis within a few weeks. Physical-chemical properties of MWCNT e.g. solubility, dispersion status etc. certainly mediate their bio-effects. However, mechanisms involved are largely unknown. Therefore, in vitro methods should be developed which allow mechanistic studies. For this present study, two types of MWCNT were used: purified (p) and COOH-functionalized (f ) MWCNT. Particle characterization was performed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and zeta potential measurement. Cultured human bronchial epithelial cell and lung fibroblast cells were exposed to p-MWCNT or f-MWCNT. Cytotoxicity was measured using the tryphan blue assay and lactate dehydrogenase (LDH) assay. Levels of collagen, an indicator of fibrogenicity, were evaluated by ELISA and western blotting. Results showed that 1) f-MWCNT was more hydrophilic than p-MWCNT due to the presence of surface attached COOH functional groups; 2) at physiologically relevant exposure concentrations (0.02-0.6 microg/cm2), f-MWCNT showed less cell damage compared to p-MWCNT in lung epithilial cells in vitro; 3) both p-MWCNT and f-MWCNT induced collagen I in fibroblast cells in vitro. Our data suggests that COOH-functionalization modulates the bio-effects of MWCNT in vitro which have important implications in nanotoxicology. [Description provided by NIOSH]
• Subjects:
  Biological Assay Chemistry Cytotoxins Environmental Monitoring Hazardous Substances Laboratories Lung Lung Diseases Microbiology Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Analytical-methods Bioassays Biological-effects Cell-damage Chemical-properties Collagen-fibrils Cytotoxic-effects Exposure-assessment Exposure-chambers Exposure-methods Fibrogenicity Fibrous-bodies Health-hazards In-vitro-study Laboratory-animals Laboratory-testing Lung-cells Lung-disorders Lung-fibrosis Microscopic-analysis Nanotechnology Particulates Physical-properties Pulmonary-disorders Respiratory-system-disorders Spectroscopes Surface-properties Toxic-materials

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  126
• NIOSHTIC Number:
  nn:20040563
• Citation:
  Toxicologist 2012 Mar; 126(Suppl 1):276
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 51st Annual Meeting and ToxExpo, March 11-15, 2012, San Francisco, California
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:25652f97e282ac4a3f340391ecd3794376d71c55e160961e5d7b2a0e521a871fc3da98d767ed71e09739334e4a54a6db70ccb38c52b52dd78e24f3026636244b
• Download URL:
  https://stacks.cdc.gov/view/cdc/193368/cdc_193368_DS1.pdf
• File Type:
  [PDF - 474.56 KB ]