Mitochondria targeting of non-peroxidizable triphenylphosphonium conjugated oleic acid protects mouse embryonic cells against apoptosis: role of cardiolipin remodeling

Details

• Personal Author:
  Greenberger JS ; Jung M-Y ; Kagan VE ; Stoyanovsky DA ; Tungekar MA ; Tyurin VA ; Tyurina YY
• Description:
  Peroxidation of cardiolipin in mitochondria is essential for the execution of apoptosis. We suggested that integration of oleic acid into cardiolipin generates non-oxidizable cardiolipin species hence protects cells against apoptosis. We synthesized mitochondria-targeted triphenylphosphonium oleic acid ester. Using lipidomics analysis we found that pretreatment of mouse embryonic cells with triphenylphosphonium oleic acid ester resulted in decreased contents of polyunsaturated cardiolipins and elevation of its species containing oleic acid residues. This caused suppression of apoptosis induced by actinomycin D. Triacsin C, an inhibitor of acyl-CoA synthase, blocked integration of oleic acid into cardiolipin and restored cell sensitivity to apoptosis. [Description provided by NIOSH]
• Subjects:
  Antigens Lipids Occupational Health Safety
• Keywords:
  Apoptosis Author Keywords: Cardiolipin Cardiolipin Oxidation Cardiolipin Remodeling Cell-biology Cell-function Cellular-function Mitochondria Mitochondria-targeted Triphenylphosphonium Oleic Acid Ester
• ISSN:
  0014-5793
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  235-241
• Volume:
  586
• Issue:
  3
• NIOSHTIC Number:
  nn:20040484
• Citation:
  FEBS Lett 2012 Feb; 586(3):235-241
• Contact Point Address:
  Yulia Y. Tyurina, Center for Free Radical and Antioxidant Health, Department of Environmental and Occupational Health, University of Pittsburgh, Bridgeside Point, 100 Technology Drive, Suite 350, Pittsburgh, PA 15219
• Email:
  yyt1@pitt.edu
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Federation of European Biochemical Societies Letters
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:98027a353580339b8c956d0da58ea480ec490999f08dc2ad1812a9577647fcbb222e64e3aacf69bd123d5217118ce76ba23b4d3b327ef81b493c716769c3729f
• Download URL:
  https://stacks.cdc.gov/view/cdc/193324/cdc_193324_DS1.pdf
• File Type:
  [PDF - 423.66 KB ]