Immunologic, spectrophotometric and nucleic acid based methods for the detection and quantification of airborne pollen

Details

• Personal Author:
  Beezhold, Donald H. ; Green, Brett J. ; Hamilton, Robert G. ; Rittenour WR
• Description:
  Microscopic identification of pollen morphological phenotypes has been the traditional method used to identify and quantify pollen collected by air monitoring stations worldwide. Although this method has enabled a semi-standardized approach for the assessment of pollen exposure, limitations including labor intensiveness, required expertise, examiner bias, and the inability to differentiate species, genera, and in some cases families have limited data derived from the these stations. Recent advances in chemical, biochemical and molecular detection methods have provided standardized alternatives to this microscopic approach. In this review, we examine the applicability of alternative methodologies, in particular nucleic acid based assays involving the quantitative polymerase chain reaction, for the standardized detection of airborne pollen. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Air Pollution, Indoor Biological Assay Environmental Monitoring Hypersensitivity Microbiology Occupational Health Particulate Matter Safety
• Keywords:
  Air-contamination Air-monitoring Airborne-particles Allergens Analytical-instruments Analytical-methods Author Keywords: Allergen Bioassays Immunoassay Immunological-tests Microscopic-analysis Morphology Nucleic-acids Pollen Polymerase Chain Reaction Quantitative-analysis Spectrophotofluorometry Standards

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• ISSN:
  0022-1759
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  47-53
• Volume:
  383
• Issue:
  1
• NIOSHTIC Number:
  nn:20040402
• Citation:
  J Immunol Methods 2012 Sep; 383(1-2):47-53
• Contact Point Address:
  Donald H. Beezhold, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute of Occupational Safety and Health, CDC, 1095 Willowdale Road, M.S. 4020, Morgantown, WV 26505, USA
• Email:
  zec1@cdc.gov
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Immunological Methods
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e3d5067f261f840fa6dfaf3642947211a175b0faef7ce266e504b1f099e7b94616eb913f093a2d8169ec7cd861dcc2b1d4753da7206df117fbe442b5f72f3281
• Download URL:
  https://stacks.cdc.gov/view/cdc/193287/cdc_193287_DS1.pdf
• File Type:
  [PDF - 315.15 KB ]