Production, characterization and utility of a panel of monoclonal antibodies 2 for the detection of toluene diisocyanate haptenated proteins

Details

• Personal Author:
  Beezhold, Donald H. ; Hettick JM ; Johnson VJ ; Ruwona TB ; Schmechel D ; Siegel, Paul D. ; Simoyi RH ; Wang W
• Description:
  Diisocyanates (dNCOs) are highly reactive low molecular weight chemicals used in the manufacture of polyurethane products and are the most commonly reported cause of occupational asthma. Mechanistic disease studies and development of biomonitoring and research tools, such as monoclonal antibodies (mAbs) have been hampered by dNCOs' ability to self- polymerize and to cross-link biomolecules. Toluene diisocyanate (TDI)-specific monoclonal antibodies (mAbs), with potential use in immunoassays for exposure and biomarker assessments, were produced and reactivities characterized against mono- and diisocyanate and dithioisocyanate protein conjugates. In general, TDI reactive mAbs displayed stronger recognition of isocyanate haptenated proteins when the NCO was in the ortho position relative to the tolyl group, and were capable of discriminating between isocyanate and isothiocyanate conjugates and between aromatic and aliphatic dNCOs. Preliminary studies using TDI vapor exposed cells suggest potential utility of these mAbs for both research and biomonitoring. [Description provided by NIOSH]
• Subjects:
  Asthma, Occupational Biological Products Environmental Monitoring Immune System Irritants Lung Lung Diseases Occupational Health Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety Workplace

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• Keywords:
  Author Keywords: TDI Biological-agents Biological-effects Biological-monitoring Biological-systems Exposure-assessment Immunologic-disorders Immunology Immunotoxins Lung-cells Lung-disorders Lung-function Monoclonal Antibody Occupational Asthma Pulmonary-system Pulmonary-system-disorders Respiratory-hypersensitivity Respiratory-irritants Respiratory-system-disorders Work-environment Workplace-monitoring Workplace-studies

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• ISSN:
  0022-1759
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  127-135
• Volume:
  373
• Issue:
  1
• NIOSHTIC Number:
  nn:20039553
• Citation:
  J Immunol Methods 2011 Oct; 373(1-2):127-135
• Contact Point Address:
  Paul D. Siegel, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505-2888
• Email:
  pds3@cdc.gov
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Immunological Methods
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6b8dfc6de220d814f1eafbfb1a13df623300b7b5c669cf45d1a6e0654b31aa4912d0f0f17ce92283dd60872df886b45095c6021777aeb0ddf9672d38899e7c88
• Download URL:
  https://stacks.cdc.gov/view/cdc/192748/cdc_192748_DS1.pdf
• File Type:
  [PDF - 1.06 MB ]