The methoxychlor metabolite, HPTE, inhibits rat luteal cell progesterone production

Details

• Personal Author:
  Akgul Y ; Derk RC ; Meighan T ; Murono EP ; Rao KMK
• Description:
  The methoxychlor metabolite, HPTE, was shown to inhibit P450-cholesterol side-chain cleavage (P450scc) activity resulting in decreased progesterone production by cultured ovarian follicular cells in previous studies. It is not known whether HPTE has any effect on progesterone formation by the corpus luteum. Results: Exposure to 100 nM HPTE reduced progesterone production by luteal cells with progressive declines to <22% of control at 500 nM HFTE. Similarly, HPTE progressively inhibited progesterone formation and P450scc catalytic activity of hCG- or 8 Br-cAMP-stimulated luteal cells. However, HPTE did not alter mRNA and protein levels of P450scc. Compounds acting as estrogen (17 beta-estradiol, bisphenol-A or octylphenol), antiestrogen (ICI) or antiandrogen (monobutyl phthalate, flutamide or M-2) added alone to luteal cells did not mimic the action of HPTE on progesterone and P450scc activity. These results suggest that HPTE directly inhibits P450scc catalytic activity resulting in reduced progesterone formation, and this action was not mediated through estrogen or androgen receptors. [Description provided by NIOSH]
• Subjects:
  Biological Products Environmental Monitoring Laboratories Occupational Health Safety Urogenital Diseases Urogenital System
• Keywords:
  Author Keywords: Methoxychlor Biological-agents Biological-monitoring Cholesterol Side-chain Cleavage Exposure-assessment HPTE Laboratory-animals Luteal Cells Ovarian Steroidogenesis P450scc Reproduction Reproductive-effects Reproductive-hazards Reproductive-system-disorders

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• ISSN:
  0890-6238
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  77-84
• Volume:
  32
• Issue:
  1
• NIOSHTIC Number:
  nn:20039311
• Citation:
  Reprod Toxicol 2011 Jul; 32(1):77-84
• Contact Point Address:
  Yucel Akgul, Department of Physiology and Pharmacology, West Virginia University School of Medicine, Morgantown, WV
• Email:
  akgulyucel@gmail.com
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Reproductive Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:cb00718e95f802696ad589aefb6059367e9e42051e2739ba8ccf45832262f0e69b46e8df38fdd11cda1114ad88cfb09d9727b2405857b5c22a99d28767908fbd
• Download URL:
  https://stacks.cdc.gov/view/cdc/192669/cdc_192669_DS1.pdf
• File Type:
  [PDF - 614.24 KB ]