Airborne endotoxin and beta-D-glucan in PM1 in agricultural and home environments

Details

• Personal Author:
  Adhikari A ; Cho KJ ; Green, Brett J. ; Grinshpun SA ; Indugula R ; Levin L ; Reponen T ; Singh U
• Description:
  Objectives: Concentrations of microbial cell wall components such as endotoxin and beta-D-glucan in airborne submicrometer particle size fraction (PM1, defined as particles with median aerodynamic diameter, da less than or equal to 1 mu m) have not been well characterized. In this study, airborne concentrations of endotoxin and beta-D-glucan among different size fractions were quantified in two distinctly different environments: farms and homes. Effect of microbial source (farm type for farms and mold damage for homes) on size-fractionated concentrations was investigated. Methods: Airborne endotoxin and beta-D-glucan were collected on 7 farms and 184 suburban homes using NIOSH two-stage sampler, a cyclone air sampler that fractionates airborne particles into three size fractions: less than or equal to 1 mu m, 1-1.8 mu m and greater than or equal to 1.8 mu m. Results: Geometric means (geometric standard deviations) of airborne total endotoxin concentration on farms and in homes were 1.20 x 10(4) (7.57) EU/m3 and 2.67 (3.82) EU/m3, respectively. Similar values of airborne total beta-D-glucan concentrations were 1.80 x 10(3) (152.88) ng/m3 and 1.91 (2.83) ng/m3, respectively. Relative proportions of PM1 endotoxin, however, were significantly higher (p < 0.001) in home samples (7.9%) than in farm samples (0.3%). Likewise, the proportion of PM1beta-D-glucan was significantly higher (p < 0.001) in homes (28.2% vs. 2.3%). Farm type significantly influenced concentrations of PM1 beta-D-glucan (p < 0.05) and total beta-D-glucan (p < 0.001). Conclusions: The results of this study demonstrate higher proportions of PM1 endotoxin and beta-D-glucan in homes compared to farms. These data further emphasize the importance of conducting size-selective air sampling for microbial exposure assessment in homes. [Description provided by NIOSH]
• Subjects:
  Aerosols Agriculture Air Pollutants, Occupational Dust Environmental Monitoring Fungi Microbiology Occupational Health Particulate Matter Safety
• Keywords:
  Aerosol-particles Aerosol-sampling Air-sampling-techniques Airborne-dusts Airborne-particles Author Keywords: Bioaerosol Cell-biology Dust-particles Dusts Farms Fine Particles Mathematical-models Microbial-test-systems Microorganisms Microscopic-analysis Models Molds Moldy Buildings Particle-aerodynamics Particulate-sampling-methods Quantitative-analysis Size-Selective Sampling

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• ISSN:
  1680-8584
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  376-386
• Volume:
  11
• Issue:
  4
• NIOSHTIC Number:
  nn:20039182
• Citation:
  Aerosol Air Qual Res 2011 Aug; 11(4):376-386
• Contact Point Address:
  Tiina Reponen, Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, OH
• Email:
  Tiina.Reponen@uc.edu
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Research Tools and Approaches: Control Technology and Personal Protective Equipment ; Healthcare and Social Assistance ; Services
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20010601
• Source Full Name:
  Aerosol and Air Quality Research
• End Date:
  20110731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:03fd7a1a5f88cd045dfbd266348c593cbe9a09733b2418dd614b7b3b780efa6f031b0d77c4771c00e12fe66d1f900438e7556e96a65d70d098e4739551bea532
• Download URL:
  https://stacks.cdc.gov/view/cdc/192621/cdc_192621_DS1.pdf
• File Type:
  [PDF - 524.61 KB ]