The effects of age and repetitive mechanical loading on systemic whole genome gene expression

Details

• Personal Author:
  Baker BA ; Cutlip, Robert G. ; Ensey JS ; Hollander MS ; Kashon ML ; Li S
• Description:
  Aging increases injury susceptibility and impairs skeletal muscle's ability to adapt to repetitive high-intensity mechanical loading. PURPOSE: The purpose of the present study was to investigate the systemic effects of these exposures on the gene expression profile in blood and if aging affects the gene response. METHODS: The left dorsiflexor muscles of young (12 wks age, N = 8) and old (30 mo age, N = 8) male Fischer 344 × Brown Norway rats were exposed 3×/week for 4.5 weeks to a protocol of 80 maximum SSCs per exposure in vivo. Animals were sacrificed 24 hours after the last exposure and RNA was isolated from the blood leukocytes. cRNA samples were prepared and loaded onto Sentrix Rat-Ref12 Beadchips (Illumina, cat#BD-27-303) following standard protocols. RESULTS: Old rats had a significantly higher expression than young rats in genes such as RANTES, ADORA2A, and IL17RA, associated with arthritis and apoptosis. Other genes involved in cell growth and proliferation were also upregulated in the old rats such as AIF1, CD59 and XDH. Conversely, genes associated with hypertrophy of normal cells, such as ALOX15, and cell death, such as CYP1B1, were downregulated in the old animals compared to the young. Significant differences were 1.5 fold or greater. CONSCLUSION: Understanding the sets of genes specifically associated with chronic mechanical loading leading to either a positive (adaptation) or negative (maladaptation) response and the effects of aging has immediate relevance for the development of new strategies that optimize muscle performance and muscle quality in aging skeletal muscle. [Description provided by NIOSH]
• Subjects:
  Age Factors Biological Factors Biomarkers Fatigue Genetics Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Age-factors Biological-factors Cell-biology Cell-function Cellular-reactions Exposure-levels Genetic-factors Immune-system Laboratory-animals Laboratory-testing Morphology Muscle-contraction Muscle-function Muscle-physiology Muscle-tension Muscles Musculoskeletal-system Musculoskeletal-system-disorders Physical-capacity Physical-reactions Physiological-effects Physiological-factors Physiological-fatigue Quantitative-analysis Repetitive-work

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• ISSN:
  0195-9131
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  42
• Issue:
  5
• NIOSHTIC Number:
  nn:20039034
• Citation:
  Med Sci Sports Exerc 2010 May; 42(5)(Suppl 1):374
• Contact Point Address:
  James S. Ensey, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, HELD, 1095 Willowdale Rd., MS H-2800, Morgantown, WV 26505
• Email:
  jee6@cdc.gov
• Federal Fiscal Year:
  2010
• Peer Reviewed:
  False
• Source Full Name:
  Medicine and Science in Sports and Exercise
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8cf9117e2e207940d8e401608fa95af759cad8ba73cd5e26bcf9326ac79e885d7f6c4018b00f9b0c237968b9fd7f18590db04954bfd398514cf4219b0729f025
• Download URL:
  https://stacks.cdc.gov/view/cdc/192527/cdc_192527_DS1.pdf
• File Type:
  [PDF - 39.11 KB ]