Comparison of quantitative airborne fungi measurements by active and passive sampling methods

Details

• Personal Author:
  Chen BT ; Lindsley, William G. ; Peccia J ; Schmechel D ; Yamamoto N
• Description:
  The present study compared the airborne fungi collection performance of a two-stage cyclone sampler (active method) to the performance of the Personal Aeroallergen Sampler (passive method) using quantitative polymerase chain reaction (qPCR) assays. Indoor air concentrations of the common fungal species Alternaria alternata, Cladosporium cladosporioides, Epicoccum nigrum, and Penicillium chrysogenum were considered. Good correlations between the two sampling methods for the fungi A. alternata, C. cladosporioides, and E. nigrum were observed and the mean effective passive sampling rates (+/-std. dev.) for these species were 0.032 (+/-0.006), 0.058 (+/-0.006), and 0.066 (+/-0.044) l min-1, respectively. Gravitational settling was the dominant collection mechanism for A. alternata and E. nigrum. The root mean square precisions for the passive sampler measurements were also comparable to those of the active sampler (49-73% and 50-102%, respectively). The passive sampler did not allow for the collection of P. chrysogenum, likely due to the insufficient gravitational settling velocity of the fungal particle with its aerodynamic diameter of less than 5 µm. The passive sampler, in conjunction with growth-independent qPCR detection methodologies, can be utilized in future exposure assessment studies to deepen our understanding of how individuals are affected by inhalation of airborne fungal pathogens and allergens, especially for those with an aerodynamic diameter greater than 5 um. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Air Pollution, Indoor Environmental Health Environmental Monitoring Hazardous Substances Microbiology Occupational Health Particulate Matter Public Health Respiratory System Risk Assessment Safety

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• Keywords:
  Air-contamination Airborne-particles Author Keywords: Fungi Bioaerosols Biohazards Exposure-assessment Gravitational Settling Indoor-air-pollution Indoor-environmental-quality Inhalation-studies Microorganisms Personal Aeroallergen Sampler (PAAS) Pollution Public-health Quantitative-analysis Quantitative Polymerase Chain Reaction (qPCR) Risk-analysis Samplers Sampling Sampling-equipment Sampling-methods Statistical-analysis Two-stage Cyclone Sampler

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• ISSN:
  0021-8502
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; OSHA Region 1 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  499-507
• Volume:
  42
• Issue:
  8
• NIOSHTIC Number:
  nn:20038866
• Citation:
  J Aerosol Sci 2011 Aug; 42(8):499-507
• Contact Point Address:
  Naomichi Yamamoto, Department of Chemical and Environmental Engineering, Yale University, New Haven, CT 06520
• Email:
  naomichi.yamamoto@yale.edu
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Healthcare and Social Assistance
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Aerosol Science
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:282a81913cc055d9bc37825116e41fd4b8f2c041eae9985f902c182a0b7f96977af2c04dc089b5d796f085814d30cc9af81b038c2c7da35b3304a8cad7849b92
• Download URL:
  https://stacks.cdc.gov/view/cdc/192413/cdc_192413_DS1.pdf
• File Type:
  [PDF - 337.83 KB ]