Increasing the ability to correctly identify latex sensitized patients using serologic tests

Details

• Personal Author:
  Accetta DJ ; Elms N ; Hoffmann RG ; Kelly KJ ; Klancnik M ; Kurup VP ; Wang, Mei Lin
• Description:
  RATIONALE: Currently, latex allergy diagnosis relies on patient history and serologic assays. The FDA-approved serologic tests have high rates of false positive results. This study investigated whether using other serologic assays would improve our ability to confidently diagnose latex allergen sensitization. METHODS: A total of 805 health care workers underwent duplicate skin testing with Clone 600 extract (FDA IND approval #4920) using previously published methodology. On the same day, serologic testing for latex specific IgE was measured by Pharmacia ImmunoCap and 4 ELISAs using latex proteins from different products (2 powdered glove extracts, clone 600 extract, and non-ammoniated latex). The values resulting in 98% and 99% specificity for each serologic test were identified. Multivariable logistic regression with backwards elimination was performed to evaluate the test results associated with an increased risk of latex allergen sensitization. RESULTS: Multivariable logistic regression using the previously reported cut-off value for the ImmunoCap assay (0.64, specificity 99%), a positive ImmunoCap was the only significant serologic factor in determining latex sensitization by skin test. CONCLUSIONS: For borderline-positive ImmunoCap assays (value 0.35-0.64) the addition of the ELISA assay with a specific powdered glove extract can increase the ability to correctly identify people with a relevant latex sensitization. [Description provided by NIOSH]
• Subjects:
  Biological Assay Diagnosis Health Personnel Hypersensitivity Occupational Health Quality Control Safety Skin Skin Diseases
• Keywords:
  Allergens Allergic-dermatitis Allergic-reactions Allergies Bioassays Diagnostic-techniques Diagnostic-tests Failure-analysis Health-care-personnel Proteins Quality-control Sensitivity-testing Sensitization Serological-techniques Serology Skin-exposure Skin-irritants Skin-sensitivity Skin-tests Statistical-analysis Surveillance

  More +
• ISSN:
  0091-6749
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; OSHA Region 5 ; West Virginia ; Wisconsin
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  127
• Issue:
  2
• NIOSHTIC Number:
  nn:20038864
• Citation:
  J Allergy Clin Immunol 2011 Feb; 127(2)(Suppl 1):AB178
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Healthcare and Social Assistance
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Allergy and Clinical Immunology
• Supplement:
  Supplement 1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:180dcf30313e63e44f7679934270447c2dd0ac1f86d38cd37afc0a71b3dde0eb7b813423c6b096156f931cbcb2cf3aba1d1266da2f4e7ad956e3376cdd365a26
• Download URL:
  https://stacks.cdc.gov/view/cdc/192411/cdc_192411_DS1.pdf
• File Type:
  [PDF - 37.58 KB ]