Pre-sampling contamination of filters used in measurements of airborne (1 --> 3)-beta-D-glucan based on glucan-specific Limulus amebocyte lysate assay

Details

• Personal Author:
  Park, Ju-Hyeong ; Shogren ES
• Description:
  Air sampling for (1 --> 3)-beta-D-glucan may be a good method for assessing inhalation exposure to airborne fungi. Pre-sampling contamination of filter media used for sampling (1 --> 3)-beta-D-glucan may lead to substantial exposure measurement errors. Using the Limulus amebocyte lysate assay, we tested for pre-sampling levels of (1 --> 3)-beta-D-glucan on three types of filters-mixed cellulose ester (MCE) [1 brand], glass fiber (GF)[1 brand], and polycarbonate (PC)[5 brands]. Levels of (1 --> 3)-beta-D-glucan on MCE filters exceeded 4586.1 pg per filter. Levels on GF filters averaged 135.3 (+/- 28.9) pg per filter (range = 94.8-160.4 pg per filter) and levels on PC filters averaged 152.4 (+/- 236.1) pg per filter (range = non-detectable-1760.7 pg per filter). Efforts to clean MCE and GF filters were unfeasible or unsuccessful. Sonicating PC filters for two hours in ethanol, followed by a wash in pyrogen-free water, effectively eliminated measured levels of (1 --> 3)-beta-D-glucan on four brands of PC filters, as compared to untreated PC filters. This pretreatment process did not appear to physically damage the PC filters. Air sampling results highlighted the potentially problematic contamination of untreated PC filters. Ensuring that sampling media are free of (1 --> 3)-beta-D-glucan before sampling is crucial to accurately measure levels of (1 --> 3)-beta-D-glucan exposure, especially in environments where levels of (1 --> 3)-beta-D-glucan are low. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Air Pollution, Indoor Biological Assay Dust Environmental Monitoring Fungi Irritants Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Air-contamination Air-samplers Air-sampling Airborne-particles Bioassays Cell-function Cellulose-fibers Dust-inhalation Dust-sampling Equipment-reliability Exposure-assessment Filters Inhalation-studies Measurement-equipment Polysaccharides Pretreatment Respiratory-irritants Respiratory-system-disorders

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• ISSN:
  1464-0325
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  13
• Issue:
  4
• NIOSHTIC Number:
  nn:20038661
• Citation:
  J Environ Monit 2011 Apr; 13(4):1082-1087
• Contact Point Address:
  Ju-Hyeong Park, National Institute for Occupational Safety and Health, Division of Respiratory Disease Studies, 1095 Willowdale Road, Morgantown, West Virginia, 26505, USA
• Email:
  gzp8@cdc.gov
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Services
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Environmental Monitoring
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1bcdf71f9c1c8fc3809e96abc1185723252971af330f8c63dcfeb0dfa8b9ec265e8e0f413f78a372e8e06d9bd1cfb9b27893f08abe527fcd1a17d36d1436cfd9
• Download URL:
  https://stacks.cdc.gov/view/cdc/192301/cdc_192301_DS1.pdf
• File Type:
  [PDF - 245.36 KB ]