Oxidative lipidomics reveals selective, but not random, pulmonary phosphilipid peroxidation after inhalation of carbon nanotubes

Details

• Personal Author:
  Amoscato A ; Fadeel B ; Kagan V ; Kapralova V ; Kisin E ; Murray A ; Shi J ; Shvedova A ; Sparvero L ; Tyurin V ; Tyurina Y
• Description:
  It is commonly accepted that lipid peroxidation is a mechanism of cell/tissue damage and death by nanoparticles, including single walled carbon nanotubes (SWCNT).We performed global ipidomics/oxidative lipidomics analysis of all molecular species in major classes of phospholipids and established that random free radical peroxidation does not happen in the lung of mice at 3 different time-points after SWCNT inhalation. C57BL/6 mice were exposed to SWCNT via inhalation (5mg/m3, whole body inhalation for 4 consecutive days, 5 h/day) and scarified 1, 7 and 28 days thereafter.We found that two most abundant and susceptible phospholipids - phosphatidylcholine and phosphatidylethanolamine - that constitute more than 75% of total phospholipids, do not undergo peroxidation at all. In addition, we demonstrated that three relatively minor anionic phospholipids - cardiolipin, phosphatidylinositol, and phosphatidylserine get oxidized in a very selective way: only few molecular species out of many in each class (10 out of 25 for CL, 3 out of 5 for PI, 1 out of 7 for PS) were converted into oxygenated species. Moreover, the selectivity was also detected towards fatty acids of the respective oxidized anionic phospholipids (not most polyunsaturated fatty acids but most abundant). We speculate that SWCNT-induced phospholipid peroxidation in lung is involved in the production of selective precursors of pro-resolving mediators of inflammation rather than in non-specific oxidative damage. [Description provided by NIOSH]
• Subjects:
  Environmental Monitoring Hazardous Substances Irritants Laboratories Lung Occupational Health Oxidants Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety

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• Keywords:
  Cell-damage Cell-function Cellular-reactions Environmental-control Injury-prevention Laboratory-animals Laboratory-testing Lung-irritants Molecular-biology Molecular-structure Nanotechnology Oxidative-metabolism Oxidative-processes Peroxidases Pulmonary-function Pulmonary-system Quantitative-analysis Respiratory-hypersensitivity Respiratory-irritants Respiratory-system-disorders Risk-analysis Risk-factors Statistical-analysis Tissue-disorders Toxins

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• ISSN:
  1096-6080
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  120
• NIOSHTIC Number:
  nn:20038512
• Citation:
  Toxicologist 2011 Mar; 120(Suppl 2):252
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  False
• Start Date:
  20050701
• Source Full Name:
  The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC
• Supplement:
  2
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c2fc33a1b0a217b2b70c00cef465d9be58cbfef70b4c802c23f225b75e7d9846f359a7b4c48c59eb2eab54f86aa7edcb4b573891aaa2c2f0379d4ecdee38c51a
• Download URL:
  https://stacks.cdc.gov/view/cdc/192198/cdc_192198_DS1.pdf
• File Type:
  [PDF - 1.09 MB ]