The use of e-cadherin immunofluorescence in pulmonary toxicologic pathology studies

Details

• Personal Author:
  Battelli LA ; Castranova, Vincent ; Friend S ; Hubbs, Ann F. ; Porter DW ; Schwegler-Berry D ; Willard P
• Description:
  E-cadherin is a calcium dependent adhesion molecule with important roles in epithelial intercellular adhesion and cellular structure. Immunofluorescent staining can localize and quantify protein expression in cells or tissues. Co-localization of 2 or more different proteins used in combination with fluorochromes of different colors can reveal the location of each protein. We investigated the use of e-cadherin immunofluorescence in different species (rat and mouse), with different fixatives (10% neutral buffered formalin, 4% paraformaldehyde, and 2.5% glutaraldehyde), with and without antigen retrieval, and as a dual label with immunofluorescence for other proteins. Mouse monoclonal anti-e-cadherin antibodies (BD Biosciences, San Jose, CA) in conjunction with fluorochrome-conjugated donkey anti-mouse antibodies clearly delineated sites of e-cadherin expression in lungs of rats or mice. EDTA heat-induced epitope retrieval was required to restore antigenicity to fixed tissues. E-cadherin could be demonstrated in formalin or paraformaldehyde fixed tissues but not in glutaraldehyde fixed tissue. Immunofluorescent double-labeling with e-cadherin can be used with immunofluorescent detection of podoplanin, a lymphatic endothelial cell marker that is also expressed by alveolar type I cells in the lung. Low levels of e-cadherin expression in type I cells allowed the double labeled type I cells to be distinguished from lymphatic endothelium and facilitated diagnosis of lymphangiectasia. E-cadherin immunofluorescent double labeling can also be used with activated caspase 3 or â-catenin immunofluorescence to localize the expression of these proteins in damaged airways. Thus, in the lung, e-cadherin immunofluorescence can demonstrate abnormal and normal epithelial cell junctions, facilitate demonstration of airway epithelial changes, and distinguish podoplaninexpressing alveolar type I cells from lymphatic endothelium. [Description provided by NIOSH]
• Subjects:
  Blood Hazardous Substances Immune System Laboratories Lung Occupational Health Respiratory Function Tests Respiratory System Safety
• Keywords:
  Biological-effects Cell-biology Cell-function Cellular-function Cellular-reactions Dose-response Exposure-levels Immune-reaction Laboratory-testing Lung-cells Lymphatic-system Lymphocytes Physiological-effects Pulmonary-function Pulmonary-system Quantitative-analysis Respiratory-function-tests Statistical-analysis Toxic-effects

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  120
• NIOSHTIC Number:
  nn:20038452
• Citation:
  Toxicologist 2011 Mar; 120(Suppl 2):123
• CAS Registry Number:
  Calcium (CAS RN 7440-70-2)
• Federal Fiscal Year:
  2011
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC
• Supplement:
  2
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b283e24751606481bec317fc00b8dad81349149cbade09bd2d7fe4a987ea5ff26172b9d9045aca697c5f57f8da50e419d340ccc8ae17eacd23c241e8a8b31616
• Download URL:
  https://stacks.cdc.gov/view/cdc/192161/cdc_192161_DS1.pdf
• File Type:
  [PDF - 1.09 MB ]