Development of a rapid ATP bioluminescence assay for biocidal susceptibility testing of rapidly growing mycobacteria

Details

• Personal Author:
  Kapoor R ; Yadav JS
• Description:
  An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The test species, Mycobacterium immunogenum, M. chelonae, and M. abscessus, showed various susceptibilities to the glutaraldehyde- and isothiazolone-based test biocides. [Description provided by NIOSH]
• Subjects:
  Aldehydes Biological Assay Disinfectants Formaldehyde Mycoses Occupational Health Safety
• Keywords:
  Adenosines Bacteria Bacterial-cultures Bioassays Biocides Cytolysis Humans Luminescence Microbial-test-systems Mycology Pharmacology Sensitivity-testing Thiazoles

  More +
• ISSN:
  0095-1137
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  48
• Issue:
  10
• NIOSHTIC Number:
  nn:20038182
• Citation:
  J Clin Microbiol 2010 Oct; 48(10):3725-3728
• Contact Point Address:
  Jagjit S. Yadav, Department of Environmental Health, University of Cincinnati, College of Medicine, Cincinnati, OH 45267-0056
• Email:
  jagjit.yadav@uc.edu
• CAS Registry Number:
  Glutaraldehyde (CAS RN 111-30-8) ; Kathon (CAS RN 55965-84-9)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Clinical Microbiology
• End Date:
  20170731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9a5fc473c258ae43de3ce10111594ca0540c07ed09b059c9be2dd9b3b5e6ee1a7892e9ca556ed9cf8261b72b6c96700eccb1cbbb3dd844f6f81c53dc16dea177
• Download URL:
  https://stacks.cdc.gov/view/cdc/191970/cdc_191970_DS1.pdf
• File Type:
  [PDF - 162.14 KB ]