Regulation of lung cancer cell migration and invasion by reactive oxygen species and caveolin-1

Details

• Personal Author:
  Chanvorachote P ; Luanpitpong S ; Nimmannit U ; Pongrakhananon V ; Rojanasakul Y ; Talbott SJ ; Wang LY
• Description:
  The acquired capability of tumor cells to migrate and invade neighboring tissues is associated with high metastatic potential and advanced stage of cancers. Recently, signaling molecules such as reactive oxygen species (ROS) and caveolin-1 (Cav-1) have been implicated in the aggressive behavior of cancer cells. However, the roles of specific ROS in cancer cell migration and Cav-1 regulation are unclear. We demonstrate here that Cav-1 plays an important role in the migration and invasion of human lung carcinoma H460 cells and that these effects are differentially regulated by cellular ROS. Using various known inhibitors and donors of ROS, we found that different ROS have different effects on Cav-1 expression and cell migration and invasion. Superoxide anion and hydrogen peroxide down-regulated Cav-1 expression and inhibited cell migration and invasion, whereas hydroxyl radical up-regulated the Cav-1 expression and promoted cell migration and invasion. The down-regulating effect of superoxide anion and hydrogen peroxide on Cav-1 is mediated through a transcription-independent mechanism that involves protein degradation via the ubiquitin-proteasome pathway. These results indicate the essential role of different ROS in cancer cell motility and through Cav-1 expression, which may provide a key mechanism controlling tumor progression and metastasis. The up-regulation of Cav-1 and cell motility by hydroxyl free radical suggests an important role of this ROS as a positive regulator of tumor progression. [Description provided by NIOSH]
• Subjects:
  Free Radicals Lung Lung Diseases Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Antitumor-agents Cancer Carcinomas Cell-alteration Cell-biology Cell-damage Cell-function Cell-migration Free-radicals Hydrogen-ion-activity Hydroxyl-groups Lung-cancer Lung-cells Molecular-biology Protein-biochemistry Proteins Tissue-culture Tumors

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• ISSN:
  0021-9258
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  285
• Issue:
  50
• NIOSHTIC Number:
  nn:20038082
• Citation:
  J Biol Chem 2010 Dec; 285(50):38832-38840
• Email:
  yrojan@hsc.wvu.edu
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Biological Chemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:48ae79e2d8abb9a054c1d8f5359c1d52323f37d8f57f20b2ac7edf5b6969af71a7277e0a63b30b4d691c3a8d50d75fe049cdab7e455d7876f3bb0ea5802ee4f9
• Download URL:
  https://stacks.cdc.gov/view/cdc/191900/cdc_191900_DS1.pdf
• File Type:
  [PDF - 1.86 MB ]