Cyanidin-3-glucoside inhibits ethanol-induced invasion of breast cancer cells overexpressing ErbB2

Details

• Personal Author:
  Bower KA ; Chen G ; Ding M ; Frank JA ; Ke ZJ ; Luo J ; Shi XL ; Wang SO ; Wang SY ; Xu M
• Description:
  BACKGROUND: Ethanol is a tumor promoter. Both epidemiological and experimental studies suggest that ethanol may enhance the metastasis of breast cancer cells. We have previously demonstrated that ethanol increased the migration/invasion of breast cancer cells expressing high levels of ErbB2. Amplification of ErbB2 is found in 20-30% of breast cancer patients and is associated with poor prognosis. We sought to identify agents that can prevent or ameliorate ethanol-induced invasion of breast cancer cells. Cyanidin-3-glucoside (C3G), an anthocyanin present in many vegetables and fruits, is a potent natural antioxidant. Ethanol exposure causes the accumulation of intracellular reactive oxygen species (ROS). This study evaluated the effect of C3G on ethanol-induced breast cancer cell migration/invasion. RESULTS: C3G attenuated ethanol-induced migration/invasion of breast cancer cells expressing high levels of ErbB2 (BT474, MDA-MB231 and MCF7(ErbB2)) in a concentration dependent manner. C3G decreased ethanol-mediated cell adhesion to the extracellular matrix (ECM) as well as the amount of focal adhesions and the formation of lamellipodial protrusion. It inhibited ethanol-stimulated phosphorylation of ErbB2, cSrc, FAK and p130(Cas), as well as interactions among these proteins. C3G abolished ethanol-mediated p130(Cas)/JNK interaction. CONCLUSIONS: C3G blocks ethanol-induced activation of the ErbB2/cSrc/FAK pathway which is necessary for cell migration/invasion. C3G may be beneficial in preventing/reducing ethanol-induced breast cancer metastasis. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Epidemiology Ethanol Neoplasms Occupational Health Safety
• Keywords:
  Alcohols Breast-cancer Cancer Cell-cultures Cell-damage Ethanols Tumors
• ISSN:
  1476-4598
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Kentucky ; Maryland ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  9
• NIOSHTIC Number:
  nn:20038002
• Citation:
  Mol Cancer 2010 Oct; 9:285
• Contact Point Address:
  Jia Luo, Department of Internal Medicine, University of Kentucky College of Medicine, Lexington, KY 40536, USA
• Email:
  jialuo888@uky.edu
• CAS Registry Number:
  Acetaldehyde (CAS RN 75-07-0)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Molecular Cancer
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0f7b491929e68dc1108a6b442a5c0ef7fc0329e68522ed4af752788a1e823fc9fd76f2a667651b06a9f6d35e3c2d57a7339de144cd1e86d9c3f085539ca53f2c
• Download URL:
  https://stacks.cdc.gov/view/cdc/191875/cdc_191875_DS1.pdf
• File Type:
  [PDF - 1.62 MB ]