Renal nitric oxide production in rat pregnancy: role of constitutive nitric oxide synthases

Details

• Personal Author:
  Baylis C ; Erdely A ; Losonczy G ; Santymire B ; Smith CA ; Venkat V ; Baylis C ; Erdely A ; Losonczy G ; Santymire B ; Smith CA ; Venkat V
• Description:
  Functional studies show that increased renal nitric oxide (NO) mediates the renal vasodilation and increased glomerular filtration rate that occur during normal pregnancy. We investigated whether changes in the constitutive NO synthases (NOS), endothelial (eNOS) and neuronal (nNOS), were associated with the increased renal NO production in normal midterm pregnancy in the rat. In kidneys from midterm pregnant (MP: 11-13 days gestation), late-term pregnant (LP: 18-20 days gestation), and similarly aged virgin (V) rats, transcript and protein abundance for eNOS and the nNOSa and nNOSß splice variants, as well as the rate of L-arginine-to-L-citrulline conversion, were determined as a measure of NOS activity. At MP, renal cortical abundance of the total eNOS protein and phosphorylated (Ser(1177)) eNOS was reduced, and L-arginine-to-L-citrulline conversion in the cortical membrane fraction was decreased; these declines were also seen in LP. There were no changes in the eNOS transcript. In contrast, L-arginine-to-L-citrulline conversion in the soluble fraction of renal cortex increased at MP and then declined at LP. This MP increase was ablated by S-methylthiocitrulline, a nNOS inhibitor. Using Western blotting, we did not detect a change in the protein abundance or transcript of the 160-kDa nNOSa, but protein abundance and transcript of the nNOSß were increased at MP in cortex. Collectively, these studies suggest that the soluble nNOSß is responsible for the increased renal cortical NO production during pregnancy. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Occupational Health Safety Urogenital System
• Keywords:
  Animal-studies Author Keywords: Endothelial Nitric Oxide Synthase Kidney-function Kidneys L-arginine-to-L-citrulline Conversion Laboratory-animals Neuronal Nitric Oxide Synthase Reproductive-system S-methylthiocitrulline Splice Variants
• ISSN:
  1931-857X
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  California ; Florida ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  299
• Issue:
  4
• NIOSHTIC Number:
  nn:20037757
• Citation:
  Am J Physiol Renal Physiol 2010 Oct; 299(4):F830-F836
• Contact Point Address:
  C. Baylis, 1600 SW Archer Rd., Univ. of Florida, POB 100274, Gainesville, FL 32667
• Email:
  baylisc@ufl.edu
• Federal Fiscal Year:
  2011
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Physiology: Renal Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8cff2b809536d076df0b05d77952869570104cd5b2e2d0304a359e92c4f14d7c72f0c3fa0a2cfc34ddafd79fd08391fb77bf18cdc6c94184ee3d01d5f21924b6
• Download URL:
  https://stacks.cdc.gov/view/cdc/191742/cdc_191742_DS1.pdf
• File Type:
  [PDF - 689.28 KB ]