Cytochrome P450 Isozymes Involved in the Metabolism of Phenol, a Benzene Metabolite

Details

• Personal Author:
  Carlson GP ; Powley MW
• Description:
  Benzene is an occupational and environmental toxicant. The major health concern for humans is acute myelogenous leukemia. To exert its toxic effects, benzene must be metabolized by cytochrome P450 to phenol and subsequently to catechol and hydroquinone. Previous research has implicated CYP2E1 in the metabolism of phenol. In this study the cytochrome P450 isozymes involved in the metabolism of phenol were examined in hepatic and pulmonary microsomes utilizing chemical inhibitors of CYP2E1, CYP2B, and CYP2F2 and using CYP2E1 knockout mice. CYP2E1 was found to be responsible for only approximately 50% of 20 microM phenol metabolism in the liver. This suggests another isozyme(s) is involved in hepatic phenol metabolism. In pulmonary microsomes both CYP2E1 and CYP2F2 were significantly involved. [Description provided by NIOSH]
• Subjects:
  Animals Benzene Energy Metabolism Environmental Exposure Environmental Health Environmental Pollution Hazardous Substances Laboratories Occupational Exposure Occupational Health Phenol Safety

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• Keywords:
  Animal-studies Benzenes Environmental-hazards Environmental-health Laboratory-animals Metabolism Metabolites Occupational-hazards Occupational-health Phenols Toxic-effects Toxins

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• ISSN:
  0378-4274
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Indiana ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  117-123
• Volume:
  125
• Issue:
  1
• NIOSHTIC Number:
  nn:20031158
• Citation:
  Toxicol Lett 2001 Dec; 125(1-3):117-123
• Contact Point Address:
  School of Health Sciences, Purdue University, 1338 Civil Engineering Building, West Lafayette, IN 47907-1338, USA
• Email:
  gcarlson@purdue.edu
• CAS Registry Number:
  Benzene (CAS RN 71-43-2)
• Federal Fiscal Year:
  2002
• Performing Organization:
  Purdue University, School of Health Sciences, West Lafayette, Indiana
• Peer Reviewed:
  True
• Start Date:
  20010701
• Source Full Name:
  Toxicology Letters
• End Date:
  20270630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:864dfc472c0dc889d04ae5621bfa2b44b8b68bc699c9773d6ff1403594428cc68943460ed51e9f5ac86634d465e0f203cca420522ca831666c32f9b9fb3e7051
• Download URL:
  https://stacks.cdc.gov/view/cdc/191493/cdc_191493_DS1.pdf
• File Type:
  [PDF - 79.58 KB ]